CA8 Matched ELISA Antibody Pair Set,Mouse

Cat: SEK50767

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CA8 Matched ELISA Antibody Pair Set,Mouse General Information

Product name
CA8 Matched ELISA Antibody Pair Set,Mouse
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Mouse CA8
Assay range
31.25-2000
Materials provided
1. Capture Antibody 1 mg/mL of rabbit anti-Mouse Carbonic Anhydrase VIII/CA8 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50767-R057)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse Carbonic Anhydrase VIII/CA8 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 50767-R001)
3. Standard Each vial contains 98 ng of recombinant Mouse Carbonic Anhydrase VIII/CA8. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
Product overview
This CA8 Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse CA8 . It contains Mouse CA8 capture antibody, Mouse CA8 detector antibody (HRP) and a highly purified E. coli-expressed recombinant Mouse CA8 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

CA8 Matched ELISA Antibody Pair Set,Mouse Images

Mouse Carbonic Anhydrase VIII/CA8 ELISA Pair Set Standard Curve 14011

CA8 Matched ELISA Antibody Pair Set,Mouse Alternative Names

AW546993 Matched ELISA Antibody Pair Set, Mouse;Ca8 Matched ELISA Antibody Pair Set, Mouse;Cals Matched ELISA Antibody Pair Set, Mouse;Cals1 Matched ELISA Antibody Pair Set, Mouse;Car8 Matched ELISA Antibody Pair Set, Mouse;Carp Matched ELISA Antibody Pair Set, Mouse;RP23-180H12.1 Matched ELISA Antibody Pair Set, Mouse;wdl Matched ELISA Antibody Pair Set, Mouse

CA8 Background Information

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.

Full Name
carbonic anhydrase VIII
References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Miyaji E, et al. (2003) Overexpression of carbonic anhydrase-related protein VIII in human colorectal cancer. The Journal of Pathology. 201(1): 37-45.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    CA8 ELISA Kit, Mouse Recombinant protein KIT50767
    info info
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