快速订购

Caspase-14 / CASP14 抗体, 兔单抗

产品数据评论相关产品实验方法
Human CASP14 抗体产品信息
免疫原:Recombinant Human Caspase-14 / CASP14 protein (Catalog#11856-H07E)
克隆号:101
Ig 类型:Rabbit IgG
浓度:
内毒素:
缓冲液:0.2 μm filtered solution in PBS with 5% trehalose
抗体制备方法:This antibody was obtained from a rabbit immunized with purified, recombinant Human Caspase-14 / CASP14 (rh Caspase-14 / CASP14; Catalog#11856-H07E; NP_036246.1; Ser2-Gln242).
Human CASP14 抗体使用指导
特异性:Human Caspase-14 / CASP14
推荐应用:ICC/IF, IF

ICC/IF: 10-25 μg/mL

储存方法:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CASP14 抗体IF实验图片
Caspase-14 / CASP14 Antibody, Rabbit MAb, Immunofluorescence
[点击查看大图]
Caption:
Immunofluorescence staining of Human CSAP14 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CASP14 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.
Other CASP14 抗体 Products
Caspase-14/CASP14 研究背景

Caspase 14 is a member of the caspase family. Caspases are a kind of cysteine proteinase consisting of a prodomain plus large and small catalytic subunits, that play a central role in cell apoptosis. Caspase 14 possesses an unusually short prodomain and is highly expressed in embryonic tissues but absent from most of the adult tissues except for the skin, which suggests a role in ontogenesis and skin physiology. Unlike the other short prodomain caspases(caspase-3, caspase-6, and caspase-7), Caspase 14 was not processed by multiple death stimuli including activation of members of the tumor necrosis factor receptor family and expression of proapaptotic members of the bcl-2 family. Caspase 14 has been described to be processed and activated by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may take part in keratinocyte terminal differentiation, which is essential for the skin barrier.

Human Caspase-14/CASP14 参考资料
  • Hu SM, et al. (1998) Caspase-14 is a novel developmentally regulated protease. The journal of biological chemistry. 273: 29648-53.
  • Marc Van De Craen, et al. (1998) Identification of a new caspase homologue: caspase-14. Cell death differ. 5(10): 838-46.
  • Product nameProduct name
    Size / Price
    货号: 11856-R101-50
    目录价: 
    单价:      (You Save: )
    产品数据可能随批次变化,最新数据请详见随货说明书。
    请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”