|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|恒河猴 ACVR2A 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||CG90059-ACG|
|恒河猴 ACVR2A 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||CG90059-ACR|
|恒河猴 ACVR2A 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||CG90059-CF|
|恒河猴 ACVR2A 基因ORF全长cDNA克隆(表达载体), C-His 标签||CG90059-CH|
|恒河猴 ACVR2A 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||CG90059-CM|
|恒河猴 ACVR2A 基因ORF全长cDNA克隆(表达载体), C-HA 标签||CG90059-CY|
|恒河猴 ACVR2A 基因ORF全长cDNA(克隆载体)||CG90059-G|
|恒河猴 ACVR2A 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||CG90059-NF|
|恒河猴 ACVR2A 基因ORF全长cDNA克隆(表达载体), N-His 标签||CG90059-NH|
|恒河猴 ACVR2A 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||CG90059-NM|
|恒河猴 ACVR2A 基因ORF全长cDNA克隆(表达载体), N-HA 标签||CG90059-NY|
|恒河猴 ACVR2A 基因ORF全长cDNA克隆(表达载体)||CG90059-UT|
ACVR2A and ACVR2B are two activin type II receptors. ACVR2A has been shown to interact with INHBA, SYNJ2BP and ACVR1B. The bovine ACVR2A gene encodes a protein of 513 amino acids which is highly homologous (approximately 98% identity) to the rat, mouse, and human ACVR2A proteins. Inactivation of ACVR2A is a common event in prostate cancer cells suggesting it may play an important role in the development of prostate cancer. The ACVR2A gene is a putative tumor suppressor gene that is frequently mutated in microsatellite-unstable colon cancers (MSI-H colon cancers). Frameshift mutation of ACVR2A may contribute to MSI-H colon tumorigenesis via disruption of alternate TGF-beta effector pathways.