|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|恒河猴 BMP2 基因全长ORF克隆 (表达载体), C端GFPSpark标签||CG90041-ACG|
|恒河猴 BMP2 基因全长ORF克隆 (表达载体), C端OFPSpark / RFP标签||CG90041-ACR|
|恒河猴 BMP2 基因全长ORF克隆 (表达载体), C端FLAG标签||CG90041-CF|
|恒河猴 BMP2 基因全长ORF克隆 (表达载体), C端His标签||CG90041-CH|
|恒河猴 BMP2 基因全长ORF克隆 (表达载体), C端Myc标签||CG90041-CM|
|恒河猴 BMP2 基因全长ORF克隆 (表达载体), C端HA标签||CG90041-CY|
|恒河猴 BMP2 基因全长ORF克隆||CG90041-G|
|恒河猴 BMP2 基因全长ORF克隆 (表达载体), N端FLAG标签||CG90041-NF|
|恒河猴 BMP2 基因全长ORF克隆 (表达载体), N端His标签||CG90041-NH|
|恒河猴 BMP2 基因全长ORF克隆 (表达载体), N端Myc标签||CG90041-NM|
|恒河猴 BMP2 基因全长ORF克隆 (表达载体), N端HA标签||CG90041-NY|
|恒河猴 BMP2 基因全长ORF克隆 (表达载体), 无标签||CG90041-UT|
BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.