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恒河猴 BMP5 基因全长ORF克隆 (表达载体), C端Myc标签

产品数据评论相关产品实验方法
Cynomolgus BMP5 cDNA Clone产品信息
NCBI注册码:XM_001109809.1
参考序列ORF长度:1365bp
cDNA基因描述:Full length Clone DNA of Macaca mulatta (Rhesus monkey) bone morphogenetic protein 5 with C terminal Myc tag.
分子别称:BMP5
分子种属:Rhesus
载体:pCMV3-C-Myc
质粒:
限制性酶切位点:
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Product nameProduct name
重组大鼠 Cripto / TDGF1 蛋白 (His 标签)重组大鼠 Latent TGF-beta 1 / TGFB1 蛋白 (His 标签)Human TGFB1 (LAP) / TGF-beta 1 Protein (His Tag)重组狗 TGF-beta 1 / TGFB1 蛋白 (His 标签)重组狗 TGFB2 / TGF-beta 2 蛋白 (His 标签)重组小鼠 TGF-beta 2 / TGFB2 蛋白 (His Tag)重组小鼠 ALK-4 / ACVR1B 蛋白 (Fc 标签)重组人 ALK-7 / ACVR1C 蛋白 (ECD, Fc 标签)重组小鼠 Latent TGF-beta 1 / TGFB1 蛋白 (His 标签)重组人 Endoglin / CD105 / ENG 蛋白 (Fc 标签)重组人 Endoglin / CD105 / ENG 蛋白 (His 标签)重组人 Decorin / DCN / SLRR1B 蛋白 (Fc 标签)重组人 Decorin / DCN / SLRR1B 蛋白 (His 标签)重组人 ALK-2 / ACVR1 蛋白 (His & Fc 标签)重组人 ALK-2 / ACVR1 / ALK2 蛋白 (His 标签)重组人 TGFBR2 蛋白 (His & Fc 标签)重组人 TGFBR1 / ALK-5 / SKR4 蛋白 (His & Fc 标签)重组人 TGFBR1 / ALK-5 / SKR4 蛋白 (aa 200-503, His & GST 标签)重组人 ALK4 / ACVR1B 蛋白 (His & Fc 标签)重组人 ALK4 / ACVR1B 蛋白 (His 标签)重组小鼠 BAMBI / NMA 蛋白 (His 标签)Rat / Mouse TGF-beta 1 / TGFB1 Protein重组人 TGFBR3 / Betaglycan 蛋白 (His 标签)重组人 Latent TGF-beta 1 / TGFB1 蛋白 (His 标签)重组人 / 恒河猴 / 狗 TGF-beta 1 / TGFB1 蛋白重组人 BAMBI / NMA 蛋白 (His 标签)重组人 Cripto / TDGF1 蛋白 (His 标签)重组人 ATF2 蛋白 (His & GST 标签)重组小鼠 ALK-2 / ACVR1 蛋白 (His & Fc 标签)重组小鼠 Endoglin / CD105 / ENG 蛋白 (His 标签)重组小鼠 TGFBR3 / Betaglycan 蛋白 (His 标签)重组小鼠 Smad2 蛋白 (His & GST 标签)重组小鼠 Smad5 蛋白 (His & GST 标签)重组小鼠 Smad5 蛋白重组小鼠 BAMBI / NMA 蛋白 (Fc 标签)重组小鼠 Smad3 蛋白 (His & GST 标签)重组狗 ALK-2 / ACVR1 / ALK2 蛋白 (Fc 标签)重组狗 ALK-2 / ACVR1 / ALK2 蛋白 (His 标签)重组大鼠 ALK-2 / ACVR1 / ALK2 蛋白 (Fc 标签)重组大鼠 Cripto / TDGF1 蛋白 (Fc 标签)重组大鼠 ACVR1B / ALK-4 蛋白 (Fc 标签)重组大鼠 TGFBR2 蛋白 (Fc 标签)重组食蟹猴 ALK-2 / ACVR1 / ALK2 蛋白 (Fc 标签)重组食蟹猴 TGFBR2 蛋白 (Fc 标签)重组食蟹猴 ACVR1B / ALK-4 蛋白 (Fc 标签)重组食蟹猴 ALK-7 / ALK7 / ACVR1C 蛋白 (Fc 标签)重组食蟹猴 TGF-beta 1 / TGFB1 蛋白 (His 标签)
研究背景

Bone Morphogenetic Protein 5 (BMP-5) is a member of the structurally and functionally related bone morphogenetic proteins (BMPs) which constitute a novel subfamily of the transforming growth factor β (TGF-β) superfamily. In agreement with a possible role in the control of cell death, BMP-5 exhibited a regulated pattern of expression in the interdigital tissue. Transcripts of BMP-5 and BMP-5 protein were abundant within the cytoplasm of the fragmenting apoptotic interdigital cells in a way suggesting that delivery of BMPs into the tissue is potentiated during apoptosis. Gain-of-function experiments demonstrated that BMP-5 has the same effect as other interdigital BMPs inducing apoptosis in the undifferentiated mesoderm and growth in the prechondrogenic mesenchyme. BMP-5 is a member of the 60A subgroup of BMPs, other members of which have been shown to stimulate dendritic growth in central and peripheral neurons. The signaling pathway that mediates the dendrite-promoting activity of BMP-5 may involve binding to BMPR-IA and activation of Smad-1, and relative levels of BMP antagonists such as noggin and follistatin may modulate BMP-5 signaling. Since BMP-5 is expressed at relatively high levels not only in the developing but also the adult nervous system, these findings suggest the possibility that BMP-5 regulates dendritic morphology not only in the developing, but also the adult nervous system. BMP-5 may play important roles not only in myocardial differentiation, but also in the formation and maintenance of endocardial cushion tissue. Additionally, high expression level of BMP-5 has been detected in certain tumors of mesenchymal origin.

参考资料
  • Yamagishi T, et al. (2001) Expression of bone morphogenetic protein-5 gene during chick heart development: possible roles in valvuloseptal endocardial cushion formation. Anat Rec. 264(4): 313-6.
  • Beck HN, et al. (2001) Bone morphogenetic protein-5 (BMP-5) promotes dendritic growth in cultured sympathetic neurons. BMC Neurosci. 2:12.
  • Zuzarte-Lus V, et al. (2004) A new role for BMP5 during limb development acting through the synergic activation of Smad and MAPK pathways. Dev Biol. 272(1): 39-52.
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    货号: CG90053-CM
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