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恒河猴 Betacellulin / BTC 基因ORF全长cDNA克隆(表达载体), C-His 标签

    产品数据评论相关产品实验方法
    食蟹猴 BTC cDNA Clone产品信息
    NCBI注册码:XM_001101880.2
    参考序列ORF长度:537bp
    cDNA基因描述:Full length Clone DNA of Macaca mulatta (Rhesus monkey) betacellulin with C terminal His tag.
    分子别称:BTC
    分子种属:Rhesus
    载体:pCMV3-C-His
    质粒:
    限制性酶切位点:
    Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    His Tag Info

    A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

    Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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    参考资料
  • Shing Y, et al. (1993) Betacellulin: a mitogen from pancreatic beta cell tumors. Science . 259(5101): 1604-7.
  • Riese DJ, et al. (1996) Betacellulin activates the epidermal growth factor receptor and erbB-4, and induces cellular response patterns distinct from those stimulated by epidermal growth factor or neuregulin-beta. Oncogene. 12(2): 345-53.
  • Bastian SE, et al. (2001) Measurement of betacellulin levels in bovine serum, colostrum and milk. J Endocrinol . 168: 203-12.
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    请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”