|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|食蟹猴 ENSA 基因全长ORF克隆 (表达载体), C端GFPSpark标签||CG90656-ACG|
|食蟹猴 ENSA 基因全长ORF克隆 (表达载体), C端OFPSpark标签||CG90656-ACR|
|食蟹猴 ENSA 基因全长ORF克隆 (表达载体), N端GFPSpark标签||CG90656-ANG|
|食蟹猴 ENSA 基因全长ORF克隆 (表达载体), N端OFPSpark标签||CG90656-ANR|
|食蟹猴 ENSA 基因全长ORF克隆 (表达载体), C端FLAG标签||CG90656-CF|
|食蟹猴 ENSA 基因全长ORF克隆 (表达载体), C端His标签||CG90656-CH|
|食蟹猴 ENSA 基因全长ORF克隆 (表达载体), C端Myc标签||CG90656-CM|
|食蟹猴 ENSA 基因全长ORF克隆 (表达载体), C端HA标签||CG90656-CY|
|食蟹猴 ENSA 基因全长ORF克隆||CG90656-G|
|食蟹猴 ENSA 基因全长ORF克隆 (表达载体), N端FLAG标签||CG90656-NF|
|食蟹猴 ENSA 基因全长ORF克隆 (表达载体), N端His标签||CG90656-NH|
|食蟹猴 ENSA 基因全长ORF克隆 (表达载体), N端Myc标签||CG90656-NM|
|食蟹猴 ENSA 基因全长ORF克隆 (表达载体), N端HA标签||CG90656-NY|
|食蟹猴 ENSA 基因全长ORF克隆 (表达载体), 无标签||CG90656-UT|
Endosulfine alpha, also known as ENSA, belongs to the endosulfine family. It is a highly conserved cAMP-regulated phosphoprotein (ARPP) family. Endosulfine alpha is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. As a protein phosphatase inhibitor, ENSA specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase By similarity. Endosulfine alpha also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.