FRZB cDNA ORF Clone, Rhesus, C-HA tag

Cat: CG90406-CY
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FRZB cDNA ORF Clone, Rhesus, C-HA tag 基本信息
基因
种属
Rhesus
NCBI 参考序列号
参考序列ORF长度
978 bp
描述
Full length Clone DNA of Rhesus frizzled-related protein with C terminal HA tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-HA
标签序列
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

FRZB Background Information

FRZB also known as sFRP-3, is a secreted protein containing a domain similar to the putative Wnt-binding region of the frizzled family of transmembrane receptors. FRZB is widely expressed in adult mammalian tissues. In the Xenopus gastrula, FRZB is regulated as a typical Spemann organizer component. FRZB also functions as a competitor for the cell-surface G-protein receptor Frizzled. It is espically important in embryonic development. Defects in FRZB gene can cause female-specific osteoarthritis (OA) susceptibility. FRZB may serve an important role in determining hip shape and may modify the relationship between hip shape and OA.

Full Name
frizzled-related protein
References
  • Hoang B., et al.,(1996), Primary structure and tissue distribution of FRZB, a novel protein related to Drosophila frizzled, suggest a role in skeletal morphogenesis. J. Biol. Chem. 271:26131-26137.
  • Mayr T., et al., (1997), Fritz: a secreted frizzled-related protein that inhibits Wnt activity.Mech. Dev. 63:109-125.
  • Leyns L., et al.,(1997), Frzb-1 is a secreted antagonist of Wnt signaling expressed in the Spemann organizer.Cell 88:747-756.
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