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食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签

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Cynomolgus GOT1 cDNA Clone产品信息
NCBI注册码:NM_001284930.1
参考序列ORF长度:1242bp
cDNA基因描述:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) Aspartate aminotransferase, cytoplasmic with N terminal Myc tag.
分子别称:cCAT, cAspAT
分子种属:Cynomolgus
载体:pCMV3-N-Myc
质粒:
限制性酶切位点:
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签 on other vectors
食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签CG90649-ACG1950
食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签CG90649-ACR1950
食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签CG90649-ANG1950
食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签CG90649-ANR1950
食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签CG90649-CF1770
食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), C-His 标签CG90649-CH1770
食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签CG90649-CM1770
食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), C-HA 标签CG90649-CY1770
食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA(克隆载体)CG90649-G760
食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签CG90649-NF1770
食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-His 标签CG90649-NH1770
食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签CG90649-NM1770
食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-HA 标签CG90649-NY1770
食蟹猴 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体)CG90649-UT1770
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研究背景

Aspartate aminotransferase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, aspartate aminotransferase and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. There is a rare in-frame deletion in aspartate aminotransferase gene, which inactivates cytosolic aspartate aminotransferase(cAST) enzyme in the Old Order Amish. This may help to understand structure and function of the enzyme and would be useful for predicting serum aspartate AST levels.

参考资料
  • Shen H, et al. (2011) Genome-wide association study identifies genetic variants in GOT1 determining serum aspartate aminotransferase levels. J Hum Genet. 56(11):801-5.
  • Doonan S, et al. (1985) Structural and genetic relationships between cytosolic and mitochondrial isoenzymes. Int J Biochem. 16(12):1193-9.
  • Panteghini M. (1990) Aspartate aminotransferase isoenzymes. Clin Biochem. 23(4):311-9.
  • Bousquet-Lemercier B, et al. (1990) Properties of human liver cytosolic aspartate aminotransferase mRNAs generated by alternative polyadenylation site selection. Biochemistry. 29(22):5293-9.
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    货号: CG90649-NM
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