|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|恒河猴 NKG2D/CD314/KLRK1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||CG90164-ACG|
|恒河猴 NKG2D/CD314/KLRK1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||CG90164-ACR|
|恒河猴 NKG2D/CD314/KLRK1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||CG90164-CF|
|恒河猴 NKG2D/CD314/KLRK1 基因ORF全长cDNA克隆(表达载体), C-His 标签||CG90164-CH|
|恒河猴 NKG2D/CD314/KLRK1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||CG90164-CM|
|恒河猴 NKG2D/CD314/KLRK1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||CG90164-CY|
|恒河猴 NKG2D/CD314/KLRK1 基因ORF全长cDNA(克隆载体)||CG90164-G|
|恒河猴 NKG2D/CD314/KLRK1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||CG90164-NF|
|恒河猴 NKG2D/CD314/KLRK1 基因ORF全长cDNA克隆(表达载体), N-His 标签||CG90164-NH|
|恒河猴 NKG2D/CD314/KLRK1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||CG90164-NM|
|恒河猴 NKG2D/CD314/KLRK1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||CG90164-NY|
|恒河猴 NKG2D/CD314/KLRK1 基因ORF全长cDNA克隆(表达载体)||CG90164-UT|
NKG2D, also known as CD314, is an immune receptor which consists of two disulphide-linked type II transmembrane proteins with short intracellular proteins uncapable to transduce signals. In order to transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.