|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|食蟹猴 Opalin / TMEM10 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||CG90667-ACG|
|食蟹猴 Opalin / TMEM10 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||CG90667-ACR|
|食蟹猴 Opalin / TMEM10 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||CG90667-CF|
|食蟹猴 Opalin / TMEM10 基因ORF全长cDNA克隆(表达载体), C-His 标签||CG90667-CH|
|食蟹猴 Opalin / TMEM10 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||CG90667-CM|
|食蟹猴 Opalin / TMEM10 基因ORF全长cDNA克隆(表达载体), C-HA 标签||CG90667-CY|
|食蟹猴 Opalin / TMEM10 基因ORF全长cDNA(克隆载体)||CG90667-G|
|食蟹猴 Opalin / TMEM10 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||CG90667-NF|
|食蟹猴 Opalin / TMEM10 基因ORF全长cDNA克隆(表达载体), N-His 标签||CG90667-NH|
|食蟹猴 Opalin / TMEM10 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||CG90667-NM|
|食蟹猴 Opalin / TMEM10 基因ORF全长cDNA克隆(表达载体), N-HA 标签||CG90667-NY|
|食蟹猴 Opalin / TMEM10 基因ORF全长cDNA克隆(表达载体)||CG90667-UT|
Opalin, or oligodendrocytic myelin paranodal and inner loop protein, is a transmembrane protein detected specifically in mammalian oligodendrocytes, and may play significant role in oligodendrocyte differentiation and myelination.Opalin has binding sites for Myt1 and cAMP-response element binding protein (CREB). Over-expression of Myt1, treatment of the cell with leukemia inhibitory factor (LIF), and cAMP analog (CREB activator) enhanced the expression of endogenous Opalin in Oli-neu cells and activated the oligodendrocyte enhancer. Thus LIF, cAMP signaling cascades and Myt1 may play significant roles in the differentiation of oligodendrocytes through their action on the Opalin oligodendrocyte enhancer. Enzymatic deglycosylation showed that myelin Opalin contained N- and O-glycans, and that the O-glycans, at least, had negatively charged sialic acids. Site-directed mutations at the glycan sites impaired the cell surface localization of Opalin. In addition to the somata and processes of oligodendrocytes, Opalin immunoreactivity was observed in myelinated axons in a spiral fashion, and was concentrated in the paranodal loop region. Immunogold electron microscopy demonstrated that Opalin was localized at particular sites in the paranodal loop membrane. These results suggest a role for highly sialylglycosylated Opalin in an intermembranous function of the myelin paranodal loops in the central nervous system.