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食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体), N-Flag 标签

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Cynomolgus PARN cDNA Clone产品信息
NCBI注册码:XM_005591297.1
参考序列ORF长度:1920bp
cDNA基因描述:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) poly(A)-specific ribonuclease with N terminal Flag tag.
分子别称:PARN
分子种属:Cynomolgus
载体:pCMV3-N-FLAG
质粒:
限制性酶切位点:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体), N-Flag 标签 on other vectors
食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签CG90462-ACG2070
食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签CG90462-ACR2070
食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签CG90462-ANG2070
食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签CG90462-ANR2070
食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体), C-Flag 标签CG90462-CF1890
食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体), C-His 标签CG90462-CH1890
食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体), C-Myc 标签CG90462-CM1890
食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体), C-HA 标签CG90462-CY1890
食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA(克隆载体)CG90462-G920
食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体), N-Flag 标签CG90462-NF1890
食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体), N-His 标签CG90462-NH1890
食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体), N-Myc 标签CG90462-NM1890
食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体), N-HA 标签CG90462-NY1890
食蟹猴 PARN/poly(A)-specific ribonuclease 基因ORF全长cDNA克隆(表达载体)CG90462-UT1890
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