Anti-Cystatin A Antibody (Mouse Monoclonal antibody) General Information
Anti-Cystatin A Antibody
Reacts with: Human
Human Cystatin A
Recombinant Human Cystatin A / CSTA protein (Catalog#10479-H07E)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cystatin A / CSTA (rh Cystatin A / CSTA; Catalog#10479-H07E; P01040; Ile2-Phe98). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #01
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Cystatin A Antibody (Mouse Monoclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-Cystatin A Antibody (Mouse Monoclonal antibody) Images
Immunochemical staining of human CSTA in human tonsil with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).
Immunochemical staining of human CSTA in human skin with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).
Immunochemical staining of human CSTA in human malignant melanoma with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).
Anti-Cystatin A Antibody Alternative Names
Anti-AREI Antibody;Anti-STF1 Antibody;Anti-STFA Antibody
Cystatin A Background Information
Cystatin-A, also known as Cystatin-AS, Stefin-A and CSTA, is a cytoplasm protein which belongs to the cystatin family. Cystatin-A / CSTA is a cysteine proteinase inhibitor with a molecular mass of 11 kDa, and is located mainly in the keratohyaline granules of the stratum granulosum and the cornified envelope of the stratum corneum in the epidermis. The cystatins are a family of cysteine protease inhibitors with homology to chicken cystatin. Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. Cystatins typically comprise about 115 amino acids, are largely acidic, contain four conserved cysteine residues known to form two disulfide bonds. Cystatins may be glycosylated and / or phosphorylated, with similarity to fetuins, kininogens, stefins, histidine-rich glycoproteins and cystatin-related proteins. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired inhibitory activity. Cystatins mainly inhibit peptidases belonging to peptidase families C1 (papain family) and C13 (legumain family).
Machleidt W., et al.,(1983), Protein inhibitors of cysteine proteinases. II. Primary structure of stefin, a cytosolic protein inhibitor of cysteine proteinases from human polymorphonuclear granulocytes. Hoppe-Seyler's Z. Physiol. Chem. 364:1481-1486.Takeda A., et al., (1989), Comparative studies on the primary structure of human cystatin as from epidermis, liver, spleen, and leukocytes.J. Biochem. 105:986-991.Kartasova T., et al.,(1987), Effects of UV, 4-NQO and TPA on gene expression in cultured human epidermal keratinocytes.Nucleic Acids Res. 15:5945-5962.