Cystatin SN Matched ELISA Antibody Pair Set,Human

Cat: SEK11568

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Cystatin SN Matched ELISA Antibody Pair Set,Human General Information

Product name
Cystatin SN Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human Cystatin SN
Assay range
62.5-4000
Materials provided
1. Capture Antibody 0.2 mg/mL of rabbit anti-CST1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 11568-R003)
2. Detection Antibody 0.25 mg/mL rabbit anti-CST1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-protector, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11568-R111)
3. Standard Each vial contains 66 ng of recombinant CST1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.
Product overview
This Cystatin SN Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human Cystatin SN . It contains Human Cystatin SN capture antibody, Human Cystatin SN detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Cystatin SN protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

Cystatin SN Matched ELISA Antibody Pair Set,Human Images

Human Cystatin SN / CST1 ELISA Pair Set Standard Curve 13936

Cystatin SN Matched ELISA Antibody Pair Set,Human Alternative Names

CST1 Matched ELISA Antibody Pair Set, Human

Cystatin SN Background Information

Cystatin-SN, also known as Cystain-SA-I, Cystatin-1, Salivary cystatin-SA-1 and CST1, is a secreted protein which belongs to the cystatin family. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. Cystatin-SN / CST1 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Cystatin-SN / CST1 is also expressed in saliva, tears, urine and seminal fluid. Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Human salivary cystatins include Cystatin-S, Cystatin-S1, Cystatin-S2, Cystatin- SA, Cystatin-SN, Cystatin-C and Cystatin-D. Cystatin-SN is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin-S, although both inhibit ficin equally well.

Full Name
cystatin SN
References
  • Saitoh E., et al.,(1987), Human cysteine-proteinase inhibitors: nucleotide sequence analysis of three members of the cystatin gene family. Gene 61:329-338.
  • Al-Hashimi I., et al., (1988), Purification, molecular cloning, and sequencing of salivary cystatin SA-1.J. Biol. Chem. 263:9381-9387.
  • Deloukas P., et al.,(2001), The DNA sequence and comparative analysis of human chromosome 20.Nature 414:865-871.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
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