Anti-Cytokeratin 14 / KRT14 Antibody

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Anti-Cytokeratin 14 / KRT14 Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-Cytokeratin 14 / KRT14 Antibody
经验证的应用
WB,IHC-P
交叉反应
Reacts with: Human, Mouse, Rat
特异性
Human Cytokeratin 14 / KRT14
免疫原
A synthetic peptide corresponding to the C-terminus of the human Cytokeratin 14.
制备方法
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the C-terminus of the human Cytokeratin 14, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Cytokeratin 14 / KRT14 Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IHC-P 1:500-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Cytokeratin 14 / KRT14 Antibody (Rabbit Polyclonal 抗体) 图片

Immunochemical staining CK14 in human skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining CK14 in human esophageal carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Anti-Cytokeratin14 rabbit polyclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:52 kDa

Observed band size:52 kDa

(We are unsure as to the identity of these extra bands.)

Immunochemical staining CK14 in mouse skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining CK14 in rat skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Anti-Cytokeratin 14 / KRT14 Antibody: 别称

Anti-CK14 Antibody; Anti-EBS3 Antibody; Anti-EBS4 Antibody; Anti-K14 Antibody; Anti-NFJ Antibody

Cytokeratin 14 / KRT14 背景信息

Cytokeratin 14, also known as Keratin 14 and K14, is a member of the keratin family. Cytokeratin 14 is a type I keratin. It is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Cytokeratin 14 is mainly expressed in the basal layer. It is also strongly expressed in the outer root sheath of anagen follicles. Cytokeratin 14 and keratin 5 may have a role in maintenance of cell proliferation potential in the basal layer of stratified epithelia, modulating phosphatidylinositol 3-kinase/Akt–mediated cell proliferation and/or Notch1-dependent cell differentiation. Cytokeratin 14 defect prevents it from working effectively with keratin 5 and interfering with the assembly of the keratin intermediate filament network. A disruption in this network makes keratinocytes fragile and prone to rupture. Minor trauma to the skin, such as rubbing or scratching, can cause these cells to break down, resulting in the formation of painful, fluid-filled blisters. Mutations in the K14 gene are also responsible for Naegeli-Franceschetti-Jadassohn syndrome and dermatopathia pigmentosa reticularis.
全称
keratin 14, type I
参考文献
  • Coulombe PA, et al., 1991, Cell. 66(6): 1301-11.
  • Schweizer J, et al., 2006, 174(2): 169-74.
  • Lugassy J, et al., 2006, Am J Hum Genet. 79(4): 724-30.
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