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大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-HA 标签

产品数据评论相关产品实验方法
E. coli FABI cDNA Clone产品信息
NCBI注册码:
参考序列ORF长度:789bp
cDNA基因描述:Full length Clone DNA of Escherichia coli enoyl-[acyl-carrier-protein] reductase, NADH-dependent with N terminal HA tag.
分子别称:envM
分子种属:E. coli
载体:pCMV3-N-HA
质粒:
限制性酶切位点:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-HA 标签 on other vectors
大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签EG30001-ACG1950
大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签EG30001-ACR1950
大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签EG30001-ANG1950
大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签EG30001-ANR1950
大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), C-Flag 标签EG30001-CF1770
大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), C-His 标签EG30001-CH1770
大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), C-Myc 标签EG30001-CM1770
大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), C-HA 标签EG30001-CY1770
大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA(克隆载体)EG30001-M760
大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-Flag 标签EG30001-NF1770
大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-His 标签EG30001-NH1770
大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-Myc 标签EG30001-NM1770
大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-HA 标签EG30001-NY1770
大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体)EG30001-UT1770
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研究背景

Enoyl-ACP reductase, also known as NADH-dependent enoyl-ACP reductase and FABI, is a cell inner membrane and peripheral membrane protein which belongs to the short-chain dehydrogenases/reductases (SDR) family and FabI subfamily. Microorganisms produce many kinds of antibiotics which function in an antagonistic capacity in nature where they have much competition. Bacterial FAS provides essential fatty acids for use in the assembly of key cellular components. Among them, FABI is an enoyl-ACP reductase which catalyzes the final and rate-limiting step of bacterial FAS. The antibiotic diazaborine interferes with the activity by binding to the protein. FABI is a potential target for selective antibacterial action, because it shows low overall sequence homology with mammalian enzymes. Various compounds have been reported as inhibitors of bacterial FabI-inhibitory compounds.

参考资料
  • Miller, WH. et al., 2002, J Med Chem  45 (15):3246-56. 
  • Kitagawa, H. et al., 2007, Bioorg Med Chem  15 (2):1106-16.
  • Zheng, C.J. et al., 2007, Biochem Biophys Res Commun 362 (4):1107-12.
  • Lu,H. et al., 2008, Acc Chem Res 41 (1):11-20.
  • Zheng,CJ. et al., 2009, J Antimicrob Chemother 63 (5):949-53.
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