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大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-Myc 标签

    产品数据评论相关产品实验方法
    大肠杆菌 FABI cDNA Clone产品信息
    NCBI注册码:
    参考序列ORF长度:789bp
    cDNA基因描述:Full length Clone DNA of Escherichia coli enoyl-[acyl-carrier-protein] reductase, NADH-dependent with N terminal Myc tag.
    分子别称:envM
    分子种属:E. coli
    载体:pCMV3-N-Myc
    质粒:
    限制性酶切位点:
    Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    Myc Tag Info

    A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

    The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-Myc 标签 on other vectors
    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签EG30001-ACG1950
    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签EG30001-ACR1950
    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签EG30001-ANG1950
    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签EG30001-ANR1950
    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), C-Flag 标签EG30001-CF1770
    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), C-His 标签EG30001-CH1770
    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), C-Myc 标签EG30001-CM1770
    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), C-HA 标签EG30001-CY1770
    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA(克隆载体)EG30001-M760
    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-Flag 标签EG30001-NF1770
    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-His 标签EG30001-NH1770
    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-Myc 标签EG30001-NM1770
    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体), N-HA 标签EG30001-NY1770
    大肠杆菌 Enoyl-ACP Reductase/FABI 基因ORF全长cDNA克隆(表达载体)EG30001-UT1770
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    研究背景

    Enoyl-ACP reductase, also known as NADH-dependent enoyl-ACP reductase and FABI, is a cell inner membrane and peripheral membrane protein which belongs to the short-chain dehydrogenases/reductases (SDR) family and FabI subfamily. Microorganisms produce many kinds of antibiotics which function in an antagonistic capacity in nature where they have much competition. Bacterial FAS provides essential fatty acids for use in the assembly of key cellular components. Among them, FABI is an enoyl-ACP reductase which catalyzes the final and rate-limiting step of bacterial FAS. The antibiotic diazaborine interferes with the activity by binding to the protein. FABI is a potential target for selective antibacterial action, because it shows low overall sequence homology with mammalian enzymes. Various compounds have been reported as inhibitors of bacterial FabI-inhibitory compounds.

    参考资料
  • Miller, WH. et al., 2002, J Med Chem  45 (15):3246-56. 
  • Kitagawa, H. et al., 2007, Bioorg Med Chem  15 (2):1106-16.
  • Zheng, C.J. et al., 2007, Biochem Biophys Res Commun 362 (4):1107-12.
  • Lu,H. et al., 2008, Acc Chem Res 41 (1):11-20.
  • Zheng,CJ. et al., 2009, J Antimicrob Chemother 63 (5):949-53.
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    货号: EG30001-NM
    目录价: 
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