EEF1B2 cDNA ORF Clone in Cloning Vector, Cynomolgus

Cat: CG90457-G
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EEF1B2 cDNA ORF Clone in Cloning Vector, Cynomolgus 基本信息
基因
种属
Cynomolgus
NCBI 参考序列号
参考序列ORF长度
678 bp
序列描述
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 210G/A, 579C/T not causing the amino acid variation. Please check the sequence information before order.
描述
Full length Clone DNA of Cynomolgus eukaryotic translation elongation factor 1 beta 2.
质粒
载体
pGEM-T Vector
测序引物
SP6 and T7 or M13-47 and RV-M
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Ampicillin
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
EEF1B2 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

EEF1B2 Background Information

EF1B, also known as EEF1B2, is a translation elongation factor. It belongs to the EF-1-beta/EF-1-delta family. Elongation factors are a set of proteins that are used in protein synthesis in the cell. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. EF1B is more complex in eukaryotes than in bacteria, and consists of three subunits: EF1B-alpha, EF1B-gamma and EF1B-beta. EF1B contains 1 GST C-terminal domain. It is involved in the transfer of aminoacylated tRNAs to the ribosome. EF1B is required to regenerate EF1A from its inactive form (EF1A-GDP) to its active form (EF1A-GTP). EF1A is then ready to interact with a new aminoacyl-tRNA to begin the cycle again.

Full Name
eukaryotic translation elongation factor 1 beta 2
References
  • Pizzuti A. et al., 1994, Biochem Biophys Res Commun. 197 (1): 154-62.
  • Rual. et al., 2005, Nature. 437 (7062): 1173-8.
  • Stelzl. et al., 2005, Cell. 122 (6): 957-68.
  • Sang Lee. et al., 2002, Biochem Biophys Res Commun. 291 (1): 158-64.
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