|Recombinant Danio rerio (zebrafish) EFNB2A / Ephrin B2a protein (Catalog#63002-Z08H)|
|0.2 μm filtered solution in PBS|
|Produced in rabbits immunized with purified, recombinant Danio rerio (zebrafish) EFNB2A / Ephrin B2a (rh EFNB2A / Ephrin B2a; Catalog#63002-Z08H; O73874; Met 1-Ala222). EFNB2A / Ephrin B2a specific IgG was purified by Danio rerio (zebrafish) EFNB2A / Ephrin B2a affinity chromatography.|
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Danio rerio (zebrafish) EFNB2A / Ephrin B2a. The detection limit for Danio rerio (zebrafish) EFNB2A / Ephrin B2a is < 0.039 ng/well.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Efnb2a, also known as ephrin B2a, belongs to the ephrin family. Members of this family are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Efnb2a binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Efnb2a, together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration.