Anti-EGFR Antibody

Cat: 10001-MM08
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All EGFR Reagents
CRO Services
Product recommended

All EGFR Reagents
Anti-EGFR Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-EGFR Antibody
Validated applications
FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human EGFR
No cross-reactivity in ELISA with Human ErbB2 Human ErbB3 Human ErbB4
Immunogen
Recombinant Human EGFR / HER1 / ErbB1 protein (Catalog#10001-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met1-Ser645). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #08
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-EGFR Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ICC-IF 1:50-1:1000  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-EGFR Antibody (Mouse Monoclonal antibody) Images
Human EGFR/HER1 Immunohistochemistry(IHC) 2938
Immunofluorescence staining of Human EGFR in A431 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human EGFR monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®594-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane.
Human EGFR/HER1 Flow Cytometry (FC) 5187
Flow cytometric analysis of Human EGFR expression on A431 cells. Cells were stained with purified anti-Human EGFR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-EGFR Antibody Alternative Names
Anti-ERBB Antibody;Anti-ERBB1 Antibody;Anti-HER1 Antibody;Anti-mENA Antibody;Anti-NISBD2 Antibody;Anti-PIG61 Antibody
EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
epidermal growth factor receptor
References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 90317-T16
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 90317-RP01
    Anti-EGFR Antibody Rabbit Polyclonal WB,ELISA,IP 80100-T52
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 80100-RP01
    Anti-EGFR Antibody Rabbit Monoclonal ELISA 51091-R004
    Anti-EGFR Antibody Rabbit Polyclonal WB,ELISA 51091-T48
    Anti-EGFR Antibody Rabbit Monoclonal ELISA 51091-R112
    Anti-EGFR Antibody Rabbit Polyclonal WB,ELISA,IP 51091-T52
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 51091-RP01
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 10001-RP01
    Anti-EGFR Antibody Rabbit Polyclonal ELISA,IHC-P 10001-T24
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 10001-RP02
    Anti-EGFR Antibody (APC) Rabbit Monoclonal FCM 10001-RE01-A
    Anti-EGFR Antibody (APC) Mouse Monoclonal FCM 10001-MM08-A
    Anti-EGFR Antibody Mouse Monoclonal ELISA 10001-MM01
    Anti-EGFR Antibody Rabbit Polyclonal WB,ELISA 10001-T48
    Anti-EGFR Antibody Rabbit Monoclonal ELISA(Cap) 10001-RE11
    Anti-EGFR Antibody Rabbit Monoclonal WB,ELISA,ELISA(Det),ICC/IF,IF 10001-R021
    Anti-EGFR Antibody (PE) Rabbit Monoclonal FCM 10001-RE01-P
    Anti-EGFR Antibody Rabbit Polyclonal WB 100372-T32
    Anti-EGFR Antibody (FITC) Rabbit Monoclonal FCM 10001-RE01-F
    Anti-EGFR Antibody Rabbit Monoclonal ELISA,FCM 10001-RE01
    Anti-EGFR Antibody Rabbit Monoclonal ELISA,IHC-P,IHC-Fr 10001-R043
    Anti-EGFR Antibody (PE) Mouse Monoclonal FCM 10001-MM08-P
    Anti-EGFR Antibody (FITC) Mouse Monoclonal FCM 10001-MM08-F
    EGFR / HER1 / ErbB1 Neutralizing Antibody Rabbit Monoclonal Neutralization 10001-REN01
    Anti-EGFR Antibody Mouse Monoclonal IHC-P 10001-MM11
    EGFR / HER1 / ErbB1 Neutralizing Antibody Rabbit Monoclonal Neutralization 10001-RE10
    info info
    添加购物车成功! 添加购物车失败!