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EGFR / HER1 / ErbB1 抗体, 鼠单抗

  • EGFR / HER1 / ErbB1 Antibody, Mouse MAb, Flow cytometric analysis
  • EGFR / HER1 / ErbB1 Antibody, Mouse MAb, Immunofluorescence
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 EGFR 抗体产品信息
免疫原:Recombinant Human EGFR / HER1 / ErbB1 protein (Catalog#10001-H08H)
克隆号:08
Ig 类型:Mouse IgG1
浓度:
内毒素:
缓冲液:0.2 μm filtered solution in PBS
抗体制备方法:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met1-Ser645). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 EGFR 抗体IF实验图片
EGFR / HER1 / ErbB1 Antibody, Mouse MAb, Immunofluorescence
[点击查看大图]
Caption:
Immunofluorescence staining of Human EGFR in A431 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human EGFR monoclonal antibody (10 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®594-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane.
 EGFR 抗体FC实验图片
EGFR / HER1 / ErbB1 Antibody, Mouse MAb, Flow cytometric analysis
[点击查看大图]
Caption:
Flow cytometric analysis of Human EGFR expression on A431 cells. Cells were stained with purified anti-Human EGFR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Other EGFR 抗体 Products
反应性: Human  
应用 : ELISA  IHC-P  IF  IHC-Fr  
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10001-R043-50
10001-R043-100
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反应性: Human  
应用 : FCM  
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10001-MM08-A-25
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反应性: Human  
应用 : FCM  
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10001-RE01-A-25
10001-RE01-A-100
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反应性: Human  
应用 : FCM  
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10001-MM08-F-25
10001-MM08-F-100
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反应性: Human  
应用 : IHC-P  
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10001-MM11-50
10001-MM11-200
10001-MM11-100
50 µg 
200 µg 
100 µg 
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反应性: Human  
应用 : ELISA  IHC-P  
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10001-T24-50
10001-T24-200
10001-T24-100
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200 µg 
100 µg 
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反应性: Human  
应用 : FCM  
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10001-MM08-P-25
10001-MM08-P-100
25 Test 
100 Tests 
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反应性: Human  
应用 : FCM  
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10001-RE01-F-25
10001-RE01-F-100
25 Test 
100 Tests 
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反应性: Human  
应用 : WB  
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100372-T32-50
100372-T32-200
100372-T32-100
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反应性: Human  
应用 : ELISA  
    10001-MM01-50
    10001-MM01-200
    10001-MM01-100
    50 µg 
    200 µg 
    100 µg 
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    反应性: Human  
    应用 : ELISA  
      10001-RP02-50
      10001-RP02-200
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      50 µg 
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      反应性: Human  
      应用 : B/N  
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      10001-REN01-500
      10001-REN01-200
      500 µg 
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      反应性: Human  
      应用 : FCM  
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      10001-RE01-P-25
      10001-RE01-P-100
      25 Test 
      100 Tests 
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      反应性: Human  
      应用 : ELISA  WB  IF  IP  ICC/IF  
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      10001-R021-50
      10001-R021-100
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      50 µg 
      100 µg 
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      反应性: Human  
      应用 : B/N  
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      10001-RE10-500
      10001-RE10-200
      500 µg 
      200 µg 
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      反应性: Human  
      应用 : 
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        10001-RE11-100
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        50 µg 
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        应用 : ELISA  
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          10001-RP01-200
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          400 µg 
          200 µg 
          100 µg 
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          反应性: Human  
          应用 : ELISA  FCM  
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          10001-RE01-100
          50 µg 
          100 µg 
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          反应性: Mouse  
          应用 : ELISA  
            51091-R112-50
            51091-R112-100
            50 µg 
            100 µg 
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            反应性: Mouse  
            应用 : ELISA  WB  IP  
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            51091-T52-50
            51091-T52-200
            51091-T52-100
            50 µg 
            200 µg 
            100 µg 
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            反应性: Mouse  
            应用 : ELISA  
              51091-R004-50
              51091-R004-100
              50 µg 
              100 µg 
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              反应性: Mouse  
              应用 : ELISA  
                51091-RP01-400
                51091-RP01-200
                51091-RP01-100
                400 µg 
                200 µg 
                100 µg 
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                反应性: Rat  
                应用 : ELISA  
                  80100-RP01-200
                  80100-RP01-100
                  200 µg 
                  100 µg 
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                  反应性: Rat  
                  应用 : ELISA  WB  IP  
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                  80100-T52-50
                  80100-T52-200
                  80100-T52-100
                  50 µg 
                  200 µg 
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                  反应性: Rhesus  
                  应用 : ELISA  
                    90317-T16-50
                    90317-T16-200
                    90317-T16-100
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                    200 µg 
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                    应用 : ELISA  
                      90317-RP01-400
                      90317-RP01-200
                      90317-RP01-100
                      400 µg 
                      200 µg 
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                      EGFR/HER1 研究背景

                      As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

                      Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

                       EGFR/HER1 参考资料
                    • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
                    • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
                    • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
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                      Size / Price
                      货号: 10001-MM08-50
                      目录价: 
                      单价:      (You Save: )

                      Datasheet & Documentation

                      产品数据可能随批次变化,最新数据请详见随货说明书。
                      请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”