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ERK2 / MAPK1 / MAPK2  蛋白,抗体,试剂盒,cDNA克隆

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ERK2 / MAPK1 / MAPK2 相关研究领域

ERK2 / MAPK1 / MAPK2 概述&蛋白信息

ERK2 / MAPK1 / MAPK2 研究背景

基因概述: The protein encoded by MAPK1 gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and ERK2 are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of ERK2 requires its phosphorylation by upstream kinases. Upon activation, ERK2 translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for MAPK1 gene.
General information above from NCBI
催化活性: ATP + a protein = ADP + a phosphoprotein.
辅因子: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
酶调控: ENZYME REGULATION: Phosphorylated by MAP2K1/MEK1 and MAP2K2/MEK2 on Thr-185 and Tyr-187 in response to external stimuli like insulin or NGF. Both phosphorylations are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates. Phosphorylation on Ser-29 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. Dephosphorylated and inactivated by DUSP3, DUSP6 and DUSP9. Inactivated by pyrimidylpyrrole inhibitors. {ECO:0000269|PubMed:12356731, ECO:0000269|PubMed:19060905}.
亚单位结构: Binds both upstream activators and downstream substrates in multimolecular complexes. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with ADAM15, ARHGEF2, ARRB2, DAPK1 (via death domain), HSF4, IER3, IPO7, DUSP6, NISCH, SGK1, and isoform 1 of NEK2. Interacts (phosphorylated form) with CAV2 ('Tyr-19'-phosphorylated form); the interaction, promoted by insulin, leads to nuclear location and MAPK1 activation. Interacts with MORG1, PEA15 and MKNK2 (By similarity). MKNK2 isoform 1 binding prevents from dephosphorylation and inactivation (By similarity). Interacts with DCC (By similarity). The phosphorylated form interacts with PML (isoform PML-4). Interacts with STYX. {ECO:0000250, ECO:0000269|PubMed:11912194, ECO:0000269|PubMed:12356731, ECO:0000269|PubMed:15358203, ECO:0000269|PubMed:15616583, ECO:0000269|PubMed:16139248, ECO:0000269|PubMed:16242327, ECO:0000269|PubMed:16581800, ECO:0000269|PubMed:17194451, ECO:0000269|PubMed:17300186, ECO:0000269|PubMed:18211802, ECO:0000269|PubMed:18296648, ECO:0000269|PubMed:18435604, ECO:0000269|PubMed:18760948, ECO:0000269|PubMed:18794356, ECO:0000269|PubMed:19060905, ECO:0000269|PubMed:19447520, ECO:0000269|PubMed:19827834, ECO:0000269|PubMed:22033920, ECO:0000269|PubMed:23847209, ECO:0000269|PubMed:8794306, ECO:0000269|PubMed:9596579, ECO:0000269|PubMed:9827991}.
结构域: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
亚细胞定位: Cytoplasm, cytoskeleton, spindle {ECO:0000250}. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Note=Associated with the spindle during prometaphase and metaphase (By similarity). PEA15-binding and phosphorylated DAPK1 promote its cytoplasmic retention. Phosphorylation at Ser- 246 and Ser-248 as well as autophosphorylation at Thr-190 promote nuclear localization. {ECO:0000250}.
翻译后修饰: Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Undergoes regulatory phosphorylation on additional residues such as Ser-246 and Ser-248 in the kinase insert domain (KID) These phosphorylations, which are probably mediated by more than one kinase, are important for binding of MAPK1/ERK2 to importin-7 (IPO7) and its nuclear translocation. In addition, autophosphorylation of Thr-190 was shown to affect the subcellular localization of MAPK1/ERK2 as well. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-187. Phosphorylation on Ser-29 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. DUSP3 and DUSP6 dephosphorylate specifically MAPK1/ERK2 and MAPK3/ERK1 whereas DUSP9 dephosphorylates a broader range of MAPKs. {ECO:0000250, ECO:0000269|PubMed:17274988, ECO:0000269|PubMed:18760948, ECO:0000269|PubMed:19053285, ECO:0000269|PubMed:19060905, ECO:0000269|PubMed:19265199, ECO:0000269|PubMed:19447520, ECO:0000269|PubMed:19494114}.; ISGylated. {ECO:0000250}.
相似的序列: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. {ECO:0000305}.; Contains 1 protein kinase domain. {ECO:0000255|PROSITE-ProRule:PRU00159}.
General information above from UniProt

ERK2 / MAPK1 / MAPK2 别称

ERK2 / MAPK1 / MAPK2 相关文献

  • Houslay MD, et al. (2003) The role of ERK2 docking and phosphorylation of PDE4 cAMP phosphodiesterase isoforms in mediating cross-talk between the cAMP and ERK signalling pathways. Biochem Soc Trans. 31(Pt 6): 1186-90.
  • Jivan A, et al. (2010) Reconstitution of the Nuclear Transport of the MAP Kinase ERK2. Methods Mol Biol. 661: 273-85.
  • Yu CG, et al. (2010) Involvement of ERK2 in traumatic spinal cord injury. J Neurochem. 113(1): 131-42.
  • Subramaniam S, et al. (2010) ERK and cell death: ERK1/2 in neuronal death. FEBS J. 277(1): 22-9.
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