Ephrin B3 Protein, Mouse, Recombinant (ECD, Fc Tag) 产品信息
> 95 % as determined by SDS-PAGE.
< 1.0 EU per μg protein as determined by the LAL method.
1.Measured by its binding ability in a functional ELISA.
2.Immobilized mouse EPHB3-His(Cat:50581-M08H) at 10μg/mL (100μL/well) can bind mouse EFNB3-Fc(Cat:51147-M02H), the EC50 of mouse EFNB3-Fc is 1-50 ng/mL.
A DNA sequence encoding the mouse Efnb3 (NP_031937.1) (Met1-Ala227) was expressed with the Fc region of human IgG1 at the C-terminus.
Predicted N Terminal
The recombinant mouse Efnb3 consists 438 amino acids and predicts a molecular mass of 48.8 kDa.
Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us
for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Ephrin B3 Protein, Mouse, Recombinant (ECD, Fc Tag) in SDS-PAGE
Ephrin B3 Protein, Mouse, Recombinant (ECD, Fc Tag) Alternative Names
EFL-6 Protein, Mouse;ELF-3 Protein, Mouse;Elk-L3 Protein, Mouse;Epl8 Protein, Mouse;LERK-8 Protein, Mouse;NLERK-2 Protein, Mouse
Ephrin B3 Background Information
Ephrin B3 belongs to the ephrin family. Ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin B3 is important in brain development as well as in its maintenance. It is especially important for forebrain function since its expression levels were particularly high in several forebrain subregions compared to other brain subregions. Ephrin B3 binds to, and induce the collapse of, commissural axons/growth cones in vitro. It may play a role in constraining the orientation of longitudinally projecting axons.
Takemoto M, et al. (2002) Ephrin-B3-EphA4 interactions regulate the growth of specific thalamocortical axon populations in vitro. Eur J Neurosci. 16(6):1168-72.Brckner K, et al. (1999) EphrinB ligands recruit GRIP family PDZ adaptor proteins into raft membrane microdomains. Neuron. 22(3):511-24.Bergemann A, et al. (1998) Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube. Oncogene. 16(4):471-80.