Flt3 Ligand Protein, Human, Recombinant (His Tag)

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Flt3 Ligand Protein, Human, Recombinant (His Tag): 产品信息

纯度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured in a cell proliferation assay using BaF3 mouse pro­B cells transfected with mouse Flt­3. The ED50 for this effect is typically 2-11 ng/mL.
蛋白构建
A DNA sequence encoding the human FLT3LG (P49771-1) (Thr27-Pro185) was expressed, with a polyhistidine tag at the N-terminus.
NCBI Reference No.
表达宿主
Baculovirus-Insect Cells
种属
Human
预测 N 端
His
分子量
The recombinant human human FLT3LG consists of 175 amino acids and predicts a molecular mass of 20.2 KDa. It migrates as an approximately 27 KDa band in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Flt3 Ligand Protein, Human, Recombinant (His Tag): 图片

Flt3 Ligand Protein, Human, Recombinant (His Tag): 别称

FL Protein, Human; FLT3L Protein, Human; FLT3LG Protein, Human

Flt3 Ligand 背景信息

FLT3L, also known as flt3 ligand, is a small molecule that acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. In vivo, FLT3L also induces the mobilization of the hematopoietic progenitors and stem cells. This may help the system to kill cancer cells. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3L controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 -positive classical DCs and their CD13 -positive tissue counterparts.
全称
fms related tyrosine kinase 3 ligand
参考文献
  • Hannum C, et al. (1994) Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs. Nature 368 (6472): 643-8.
  • Lyman SD, et al. (1995) Identification of soluble and membrane-bound isoforms of the murine flt3 ligand generated by alternative splicing of mRNAs. Oncogene 10 (1): 149-57.
  • Lyman SD, et al. (1994) Molecular cloning of a ligand for the flt3/flk-2 tyrosine kinase receptor: a proliferative factor for primitive hematopoietic cells. Cell 75 (6): 1157-67.
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