Anti-FSTL1 Antibody

Cat: 10924-R009

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All FSTL1 Reagents

Anti-FSTL1 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-FSTL1 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human FSTL1
Recombinant Human FSTL1 protein (Catalog#10924-H08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Human FSTL1 (rh FSTL1; Catalog#10924-H08H; NP_009016.1; Met1-Ile308).
Monoclonal Rabbit IgG Clone #009
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-FSTL1 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution Notes
ICC-IF 1:20-1:100  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-FSTL1 Antibody (Rabbit Monoclonal antibody) Images

Human FSTL1 Flow Cytometry (FC) 5172
Flow cytometric analysis of Human FSTL1 expression on HeLa cells . The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human FSTL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Human FSTL1 Immunohistochemistry(IHC) 5470
Confocal immunofluorescence staining of Human FSTL1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FSTL1 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green), countstained with DAPI (blue).Positive staining was localized to cytoplasm.

Anti-FSTL1 Antibody Alternative Names

Anti-FRP Antibody;Anti-FSL1 Antibody;Anti-MIR198 Antibody

FSTL1 Background Information

Follistatin-related protein 1 (FSTL1)  is an extracellular glycoprotein whose functional significance in physiological and pathological processes is incompletely understood. Recently, we have shown that FSTL1 acts as a muscle-derived secreted factor that is up-regulated by Akt activation and ischemic stress and that FSTL1 exerts favorable actions on the heart and vasculature. Here, we sought to identify the receptor that mediates the cellular actions of FSTL1. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. FSTL1 is thought to be an autoantigen associated with rheumatoid arthritis. DIP2A functions as a novel receptor that mediates the cardiovascular protective effects of FSTL1. Experiment results have provided in vivo and in vitro evidence to demonstrate that Fstl1 modulates lung development and alveolar maturation, in part, through BMP4 signaling.

Full Name
follistatin-like 1
  • Rosenberg MI, et al. (2006) MyoD inhibits Fstl1 and Utrn expression by inducing transcription of miR-206. J Cell Biol. 175(1): 77-85.
  • Ouchi N, et al. (2010) DIP2A functions as a FSTL1 receptor. J Biol Chem. 285(10): 7127-34.
  • Geng Y, et al. (2011) Follistatin-like 1 (Fstl1) is a bone morphogenetic protein (BMP) 4 signaling antagonist in controlling mouse lung development. Proc Natl Acad Sci U S A. 108(17): 7058-63.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-FSTL1 Antibody Rabbit Polyclonal ELISA 80885-RP01
    Anti-FSTL1 Antibody Mouse Monoclonal ELISA 80885-MM12
    Anti-FSTL1 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 80885-T60
    Anti-FSTL1 Antibody Rabbit Polyclonal ELISA 51127-RP01
    Anti-FSTL1 Antibody Rabbit Polyclonal WB,ELISA 51127-T48
    Anti-FSTL1 Antibody Rabbit Polyclonal WB,ELISA,IHC-P 51127-T56
    Anti-FSTL1 Antibody Mouse Monoclonal ELISA 10924-MM09
    Anti-FSTL1 Antibody (FITC) Rabbit Monoclonal FCM 10924-R009-F
    Anti-FSTL1 Antibody Rabbit Monoclonal ICC/IF,IF 10924-R026
    Anti-FSTL1 Antibody Rabbit Polyclonal ELISA 10924-RP02
    Anti-FSTL1 Antibody Rabbit Polyclonal ELISA 10924-RP01
    Anti-FSTL1 Antibody Rabbit Monoclonal ELISA 80885-R011
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