快速订购

Text Size:AAA

人 FSTL5 基因ORF全长cDNA(克隆载体)

产品数据评论相关产品实验方法
Human FSTL5 cDNA Clone产品信息
NCBI注册码:NM_020116.3
参考序列ORF长度:2544bp
cDNA基因描述:Full length Clone DNA of Homo sapiens follistatin-like 5.
分子别称:FSTL5
分子种属:Human
载体:pGEM-T Vector
质粒:pGEM-FSTL5
限制性酶切位点:
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:SP6 and T7 or M13-47 and RV-M
启动子:
Application:
抗生素(大肠杆菌):Ampicilin
抗生素(哺乳动物细胞):
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Product nameProduct name
研究背景

FSTL5 may have molecular function (calcium ion binding) and to localize in various compartments (cytoplasm, extracellular space, extracellular region). FSTL5 expression denoted a dismal prognosis both within and across medulloblastoma subgroups. FSTL5 gene is well expressed, 1.0 times the average gene in this release. The sequence of this gene is defined by 120 GenBank accessions from 113 cDNA clones, some from brain, cerebellum, eye, melanotic melanoma, skin, amygdala, breast and 24 other tissues. FSTL5 gene contains 27 distinct introns. The addition of FSTL5 immunohistochemistry to existing molecular stratification schemes constitutes a reliable and cost-effective tool for prognostication in future clinical trials of medulloblastoma.

参考资料
  • Masuda T, et al. (2009) Laser capture microdissection and cDNA array analysis for identification of mouse KIAA/FLJ genes differentially expressed in the embryonic dorsal spinal cord. Brain Res. 1249:61-7.
  • Kingwell K. (2011) FSTL5--a new prognostic biomarker for medulloblastoma. Nat Rev Neurol. 7(11):598.
  • Remke M, et al. (2011) FSTL5 is a marker of poor prognosis in non-WNT/non-SHH medulloblastoma. J Clin Oncol. 29(29):3852-61.
  • Size / Price
    货号: HG13408-G
    目录价: 
    单价:      (You Save: )
    规格:
    数量:+-
    库存In Stock
    大包装查询加入购物车
    Contact Us
    产品图片
        您曾浏览过的产品
          产品数据可能随批次变化,最新数据请详见随货说明书。
          请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”