Fractalkine Protein, Human, Recombinant (His Tag)

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Fractalkine Protein, Human, Recombinant (His Tag): 产品信息

纯度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its ability to compete with biotinylated recombinant human CX3CL1-His for binding to immobilized recombinant mouse CD4-his (Cat:50134-M08H)in a functional ELISA.
蛋白构建
A DNA sequence encoding the human CX3CL1 (NP_002987.1) extracellular domain (Met 1-Arg 339) with a C-terminal polyhistidine tag was expressed.
NP No.
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Gln 25
分子量
The secreted recombinant human CX3CL1 comprises 329 amino acids with a predicted molecular mass of 35 kDa. As a result of different glycosylation, rhCX3CL1 migrates between 50-70 kDa in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Fractalkine Protein, Human, Recombinant (His Tag): 图片

Fractalkine Protein, Human, Recombinant (His Tag): 别称

ABCD-3 Protein, Human; C3Xkine Protein, Human; CXC3 Protein, Human; CXC3C Protein, Human; fractalkine Protein, Human; neurotactin Protein, Human; NTN Protein, Human; NTT Protein, Human; SCYD1 Protein, Human

Fractalkine 背景信息

Fractalkine or Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a member of the CX3C chemokine family. Fractalkine / CX3CL1 is a unique chemokine that functions not only as a chemoattractant but also as an adhesion molecule and is expressed on endothelial cells activated by proinflammatory cytokines, such as interferon-gamma and tumor necrosis factor-alpha. Fractalkine/CX3CL1 is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Fractalkine / CX3CL1 is associated with dendritic cells (DC) in epidermis and lymphoid organs. The fractalkine receptor, CX3CR1, is expressed on cytotoxic effector lymphocytes, including natural killer (NK) cells and cytotoxic T lymphocytes, which contain high levels of intracellular perforin and granzyme B, and on macrophages. Soluble fractalkine causes migration of NK cells, cytotoxic T lymphocytes, and macrophages, whereas the membrane-bound form captures and enhances the subsequent migration of these cells in response to secondary stimulation with other chemokines.
全称
chemokine (C-X3-C motif) ligand 1
参考文献
  • Imai T, et al. (1997) Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell. 91(4): 521-30.
  • Papadopoulos EJ, et al. (1999) Fractalkine, a CX3C chemokine, is expressed by dendritic cells and is up-regulated upon dendritic cell maturation. Eur J Immunol. 29 (8): 2551-9.
  • Umehara H, et al. (2004) Fractalkine in vascular biology: from basic research to clinical disease". Arterioscler. Thromb Vasc Biol. 24 (1): 34-40.
  • P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy
    Author
    Wang, H;Hong, LJ;Huang, JY;Jiang, Q;Tao, RR;Tan, C;Lu, NN;Wang, CK;Ahmed, MM;Lu, YM;Liu, ZR;Shi, WX;Lai, EY;Wilcox, CS;Han, F;
    Year
    2015
    Journal
    Cell Research
    Application
    cell-based
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