GAPDH Protein, Mouse, Recombinant (His Tag)

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GAPDH Protein, Mouse, Recombinant (His Tag): 产品信息

纯度
> 85 % as determined by SDS-PAGE
内毒素
Please contact us for more information.
生物活性
Testing in progress
蛋白构建
A DNA sequence encoding the mouse GAPDH (P16858) (Met1-Glu333) was expressed with a polyhistidine tag at the N-terminus.
NCBI Reference No.
表达宿主
E. coli
种属
Mouse
预测 N 端
His
分子量
The recombinant mouse GAPDH consists of 349 amino acids and predicts a molecular mass of 37.9 KDa. It migrates as an approximately 38 KDa band in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液
Lyophilized from sterile 50 mM Tris, 200 mM Arg, 10% gLycerol.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

GAPDH Protein, Mouse, Recombinant (His Tag): 图片

GAPDH Protein, Mouse, Recombinant (His Tag): 别称

Gapd Protein, Mouse

GAPDH 背景信息

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.
全称
glyceraldehyde-3-phosphate dehydrogenase
参考文献
  • Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  • Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  • Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  • Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
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