Anti-Intrinsic Factor Antibody

Cat: 13544-RP02
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All Intrinsic Factor Reagents
Anti-Intrinsic Factor Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Intrinsic Factor Antibody
Validated applications
ELISA,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human Intrinsic Factor
Immunogen
Recombinant Human GIF / Gastric intrinsic factor protein (Catalog#13544-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human GIF / Gastric intrinsic factor (rh GIF / Gastric intrinsic factor; Catalog#13544-H08H; P27352-1; Met1-Tyr417). GIF / Gastric intrinsic factor specific IgG was purified by Human GIF / Gastric intrinsic factor affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Intrinsic Factor Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  
IHC-P 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Intrinsic Factor Antibody (Rabbit Polyclonal antibody) Images
Human Gastric intrinsic factor / GIF Immunohistochemistry(IHC) 4139
Immunochemical staining of human GIF in human stomach with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to parietal cells.
Anti-Intrinsic Factor Antibody Alternative Names
Anti-IF Antibody;Anti-IFMH Antibody;Anti-INF Antibody;Anti-TCN3 Antibody
Intrinsic Factor Background Information

Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver.

Full Name
gastric intrinsic factor (vitamin B synthesis)
References
  • Gerdin AK. (2010) The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice. Acta Opthalmologica. 88:925-7.
  • AU - Berlin H, et al. (1968) ORAL TREATMENT OF PERNICIOUS ANEMIA WITH HIGH DOSES OF VITAMIN B12 WITHOUT INTRINSIC FACTOR. Acta Medica Scandinavica. 184(1-6):247-58.
  • Hewitt JE, et al. (1991) Human gastric intrinsic factor: characterization of cDNA and genomic clones and localization to human chromosome 11. Genomics. 10(2):432-40.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Intrinsic Factor Antibody Rabbit Monoclonal WB,IP 13544-R012
    Anti-Intrinsic Factor Antibody Rabbit Monoclonal ELISA,ELISA(Cap) 13544-R016
    Anti-Intrinsic Factor Antibody Rabbit Polyclonal ELISA 13544-RP01
    Anti-Intrinsic Factor Antibody Rabbit Monoclonal FCM 13544-R007
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