The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|人 RACK1/GNB2L1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG16196-ACG|
|人 RACK1/GNB2L1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG16196-ACR|
|人 RACK1/GNB2L1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG16196-CF|
|人 RACK1/GNB2L1 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG16196-CH|
|人 RACK1/GNB2L1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG16196-CM|
|人 RACK1/GNB2L1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG16196-CY|
|人 RACK1/GNB2L1 基因ORF全长cDNA(克隆载体)||HG16196-G|
|人 RACK1/GNB2L1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG16196-NF|
|人 RACK1/GNB2L1 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG16196-NH|
|人 RACK1/GNB2L1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG16196-NM|
|人 RACK1/GNB2L1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG16196-NY|
|人 RACK1/GNB2L1 基因ORF全长cDNA克隆(表达载体)||HG16196-UT|
RACK1 (guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1), also known as GNB2L1, contains 7 WD repeats and belongs to the WD repeat G protein beta family. In the liver, RACK1 is expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. It is up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue. RACK1 is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. It interacts with a wide variety of proteins and plays a role in many cellular processes. GNB2L1 binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. RACK1 may recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. It inhibits the activity of SRC kinases including SRC, LCK and YES1. RACK1 also inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. It enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer.