快速订购

甲型流感 H3N2 (A/Aichi/2/1968) 神经氨酸酶 (Neuraminidase / NA) (His 标签)

  • Influenza A H3N2 (A/Aichi/2/1968) Neuraminidase / NA (His Tag) SDS-PAGE
产品数据评论相关产品实验方法
H3N2 NA 蛋白产品信息
分子别称:NA
蛋白构建:A DNA sequence encoding the Influenza A virus (A/Aichi/2/1968(H3N2)) neuraminidase (Q75VQ4.1) (His36-Ile469) was expressed with a N-terminal polyhistidine tag.
分子种属:H3N2
表达宿主:Human Cells
运输说明:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
H3N2 NA 蛋白质控检测
纯度:> 90 % as determined by SDS-PAGE
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
稳定性:Samples are stable for up to twelve months from date of receipt at -70℃
预测N端:His
分子量:The recombinant neuraminidase of Influenza A virus (A/Aichi/2/1968 (H3N2)) comprises 453 amino acids and has a predicted molecular mass of 50.8 kDa. The apparent molecular mass of the protein is approximately 76.8 kDa in SDS-PAGE under reducing conditions.
缓冲液:Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
H3N2 NA 蛋白使用指导
储存方法:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶条件:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
H3N2 NA 蛋白SDS-PAGE
Influenza A H3N2 (A/Aichi/2/1968) Neuraminidase / NA (His Tag) SDS-PAGE

NA 相关信号通路

    Other NA 蛋白 Products
    表达宿主: Human Cells  
    • Slide 1
    40197-V07H-50
    40197-V07H-100
    50 µg 
    100 µg 
    Add to Cart
    描述: Active  
    表达宿主: Human Cells  
    • Slide 1
    11058-V01H-50
    11058-V01H-100
    11058-V01H-10
    50 µg 
    100 µg 
    10 µg 
    Add to Cart
    描述: Active  
    表达宿主: Human Cells  
      11058-VNAHC-200
      11058-VNAHC-100
      200 U 
      100 U 
      Add to Cart
      描述: Active  
      表达宿主: Human Cells  
        11058-VNAHC1-200
        200 U 
        Add to Cart
        表达宿主: Human Cells  
        • Slide 1
        40064-V07H-B-50
        40064-V07H-B-100
        40064-V07H-B-10
        50 µg 
        100 µg 
        10 µg 
        Add to Cart
        表达宿主: Human Cells  
        • Slide 1
        40064-V07H-50
        40064-V07H-100
        40064-V07H-10
        50 µg 
        100 µg 
        10 µg 
        Add to Cart
        描述: Active  
        表达宿主: Human Cells  
          11676-VNAHC-800
          11676-VNAHC-60
          800 U 
          60 U 
          Add to Cart
          表达宿主: Human Cells  
          • Slide 1
          40202-V07H-50
          40202-V07H-100
          50 µg 
          100 µg 
          Add to Cart
          表达宿主: Human Cells  
            40202-VNAHC-1000
            40202-VNAHC-100
            1000 U 
            100 U 
            Add to Cart
            表达宿主: Human Cells  
            • Slide 1
            40034-V07H-50
            40034-V07H-100
            40034-V07H-10
            50 µg 
            100 µg 
            10 µg 
            Add to Cart
            表达宿主: Human Cells  
            • Slide 1
            40040-V07H-50
            40040-V07H-100
            50 µg 
            100 µg 
            Add to Cart
            Neuraminidase 研究背景

            Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell.

            Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our own cell surface proteins.

            Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.

            Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. Monoclonal or polyclonal antibody can be raised with protein based antigen or peptide based antigen. Antibody raised with protein based antigen could have better specificity and/or binding affinity than antibody raised with peptide based antigen, but cost associated with the recombinant protein antigen is usually higher. Anti influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection.

            Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.

            H3N2 Neuraminidase 参考资料
          • Sardet C., et al.,(1989), Molecular cloning, primary structure, and expression of the human growth factor-activatable Na+/H+ antiporter. Cell 56:271-280.
          • Sardet C., et al., (1990), Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of 110 kD.Science 247:723-726.
          • Tse C.-M., et al.,(1991), Molecular cloning and expression of a cDNA encoding the rabbit ileal villus cell basolateral membrane Na+/H+ exchanger.EMBO J. 10:1957-1967.
          • Product nameProduct name
            Size / Price
            货号: 40199-V07H-10
            目录价: 
            单价:      (You Save: )

            Datasheet & Documentation

            Contact Us
            产品数据可能随批次变化,最新数据请详见随货说明书。
            请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”