The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|恒河猴 HSPA8/HSC70 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||CG90471-ACG|
|恒河猴 HSPA8/HSC70 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||CG90471-ACR|
|恒河猴 HSPA8/HSC70 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||CG90471-CF|
|恒河猴 HSPA8/HSC70 基因ORF全长cDNA克隆(表达载体), C-His 标签||CG90471-CH|
|恒河猴 HSPA8/HSC70 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||CG90471-CM|
|恒河猴 HSPA8/HSC70 基因ORF全长cDNA克隆(表达载体), C-HA 标签||CG90471-CY|
|恒河猴 HSPA8/HSC70 基因ORF全长cDNA(克隆载体)||CG90471-G|
|恒河猴 HSPA8/HSC70 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||CG90471-NF|
|恒河猴 HSPA8/HSC70 基因ORF全长cDNA克隆(表达载体), N-His 标签||CG90471-NH|
|恒河猴 HSPA8/HSC70 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||CG90471-NM|
|恒河猴 HSPA8/HSC70 基因ORF全长cDNA克隆(表达载体), N-HA 标签||CG90471-NY|
|恒河猴 HSPA8/HSC70 基因ORF全长cDNA克隆(表达载体)||CG90471-UT|
HSPA8, also known as HSC70, is a member of the heat shock protein family due to homology with other heat shock proteins. The heat shock protein 70 family is comprised by both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 belongs to the heat-shock cognate subgroup. Members of the human heat-shock protein multigene family have several highly conserved proteins with structural and functional properties in common, but vary in the extent of their inducibility in response to metabolic stress. HSPA8 is constitutively expressed and performs functions related to normal cellular processes. This protein binds to nascent polypeptides to facilitate correct protein folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. HSPA8 acts as a repressor of transcriptional activation. It inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones. It also is a ATPase that works with auxilin to remove clathrin coated vesicles. In neurons, synaptojanin is also an important protein involved in vesicle uncoating.