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人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体)

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     AARS cDNA Clone产品信息
    NCBI注册码:NM_001605.2
    参考序列ORF长度:2907bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens alanyl-tRNA synthetase.
    分子别称:AARS
    分子种属:Human
    载体:pCMV3-untagged
    质粒:
    限制性酶切位点:
    Tag序列:
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with AARS qPCR primers for gene expression analysis, HP100828 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Ampicillin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体) on other vectors
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG10951-ACG2550
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG10951-ACR2550
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG10951-ANG2550
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG10951-ANR2550
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10951-CF2370
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体), C-His 标签HG10951-CH2370
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG10951-CM2370
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG10951-CY2370
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA(克隆载体)HG10951-M1160
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体)HG10951-M-N2370
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG10951-NF2370
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体), N-His 标签HG10951-NH2370
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG10951-NM2370
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG10951-NY2370
    人 AARS / alanyl-tRNA synthetase 基因ORF全长cDNA克隆(表达载体)HG10951-UT2370
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    Product nameProduct name
    研究背景

    Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections. 

    参考资料
  • Wakasugi K, et al. (1999) Two Distinct Cytokines Released from a Human Aminoacyl-tRNA Synthetase. Science. 284 (5411): 147-51.
  • Sokabe M, et al. (2009) The structure of alanyl-tRNA synthetase with editing domain. Proc Natl Acad Sci . 106 (27): 11028-33.
  • Skupinska M, et al. (2009) AARS--the etiological factor and the attractive target of many disorders. Postepy Biochem. 55 (4): 373-84.
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    货号: HG10951-UT
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