|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 ACO1/irp1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10966-ACG|
|人 ACO1/irp1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10966-ACR|
|人 ACO1/irp1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG10966-ANG|
|人 ACO1/irp1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG10966-ANR|
|人 ACO1/irp1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10966-CF|
|人 ACO1/irp1 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10966-CH|
|人 ACO1/irp1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10966-CM|
|人 ACO1/irp1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10966-CY|
|人 ACO1/irp1 基因ORF全长cDNA(克隆载体)||HG10966-M|
|人 ACO1/irp1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10966-M-F|
|人 ACO1/irp1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10966-NF|
|人 ACO1/irp1 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10966-NH|
|人 ACO1/irp1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10966-NM|
|人 ACO1/irp1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10966-NY|
|人 ACO1/irp1 基因ORF全长cDNA克隆(表达载体)||HG10966-UT|
Aconitase 1(ACO1) or IRP1 is one member of the aconitase family that contains a diverse group of iron-sulphur(Fe-S) isomerases and two types of iron regulatory protein. Aconitase exits in two forms: one is soluble and the other is mitochondrial. ACO1 is the soluble existing form, and the mitochondrial form is ACO2. Residues from all three N-terminal domains and the larger C-terminal domain contribute to the active site region. When the enzyme is activated, it gains an additional iron atom. ACO1 can assume two different functions in cells, depending on different conditions. During iron scarcity or oxidative stress, ACO1 binds to mRNA stem-loop structures called iron responsive elements to modulate the translation of iron metabolism genes. In iron-rich conditions, ACO1 binds an iron-sulfur cluster to function as a cytosolic aconitase.