|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 AIM2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG11654-ACG|
|人 AIM2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG11654-ACR|
|人 AIM2 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG11654-ANG|
|人 AIM2 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG11654-ANR|
|人 AIM2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG11654-CF|
|人 AIM2 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG11654-CH|
|人 AIM2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG11654-CM|
|人 AIM2 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG11654-CY|
|人 AIM2 基因ORF全长cDNA(克隆载体)||HG11654-M|
|人 AIM2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG11654-NF|
|人 AIM2 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG11654-NH|
|人 AIM2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG11654-NM|
|人 AIM2 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG11654-NY|
|人 AIM2 基因ORF全长cDNA克隆(表达载体)||HG11654-UT|
AIM2, Absent In Melanoma 2 is a member of the interferon-inducible HIN-200 protein family that contains an amino-terminal pyrin domain and a carboxy-terminal oligonucleotide/oligosaccharide-binding domain, senses cytoplasmic DNA by means of its oligonucleotide/oligosaccharide-binding domain and interacts with ASC (apoptosis-associated speck-like protein containing a CARD) through its pyrin domain to activate caspase-1. In response to foreign cytoplasmic DNA, AIM2 forms an inflammasome, resulting in caspase activation in inflammatory cells. It had been pointed to a role of AIM2 function in both inflammation and cancer. AIM-2 antigen is expressed in a wide variety of tumor types, including neuroectodermal tumors, as well as breast, ovarian and colon carcinomas. AIM-2 could be used as a tumor antigen target for monitoring vaccine trials or to develop antigen specific active immunotherapy for glioma patients.