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人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体), N-His 标签

产品数据评论相关产品实验方法
Human AK4 cDNA Clone产品信息
NCBI注册码:BC016180
参考序列ORF长度:672bp
cDNA基因描述:Full length Clone DNA of Homo sapiens adenylate kinase 4 with N terminal His tag.
分子别称:AK3, AK3L1, AK3L2
分子种属:Human
载体:pCMV3-N-His
质粒:
限制性酶切位点:
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体), N-His 标签 on other vectors
人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG12406-ACG1950
人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG12406-ACR1950
人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG12406-ANG1950
人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG12406-ANR1950
人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG12406-CF1770
人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体), C-His 标签HG12406-CH1770
人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG12406-CM1770
人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG12406-CY1770
人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA(克隆载体)HG12406-G760
人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG12406-NF1770
人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体), N-His 标签HG12406-NH1770
人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG12406-NM1770
人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG12406-NY1770
人 AK4 / Adenylate Kinase 4 / AK3L1 基因ORF全长cDNA克隆(表达载体)HG12406-UT1770
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研究背景

Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member the adenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.

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货号: HG12406-NH
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数量:+-
库存2-3 weeks
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