|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|人 ALK-3 / BMPR1A 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10446-ACG|
|人 ALK-3 / BMPR1A 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10446-ACR|
|人 ALK-3 / BMPR1A 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10446-CF|
|人 ALK-3 / BMPR1A 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10446-CH|
|人 ALK-3 / BMPR1A 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10446-CM|
|人 ALK-3 / BMPR1A 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10446-CY|
|人 ALK-3 / BMPR1A 基因ORF全长cDNA(克隆载体)||HG10446-M|
|人 ALK-3 / BMPR1A 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10446-M-F|
|人 ALK-3 / BMPR1A 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10446-NF|
|人 ALK-3 / BMPR1A 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10446-NH|
|人 ALK-3 / BMPR1A 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10446-NM|
|人 ALK-3 / BMPR1A 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10446-NY|
|人 ALK-3 / BMPR1A 基因ORF全长cDNA克隆(表达载体)||HG10446-UT|
Activin receptor-Like Kinase 3 (ALK-3), also known as Bone Morphogenetic Protein Receptor, type IA (BMPR1A), is a type I receptor for bone morphogenetic proteins (BMPs) which belong to the transforming growth factor beta (TGF-β) superfamily. The BMP receptors form a subfamily of transmembrane serine/threonine kinases including the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. ALK-3/BMPR1A is expressed in the epithelium during branching morphogenesis. Deletion of BMPR1A in the epithelium with an Sftpc-cre transgene leads to dramatic defects in lung development. ALK-3 and ALK-6 share a high degree of homology, yet possess distinct signaling roles. The transforming growth factor (TGF)-beta type III receptor (TbetaRIII) enhanced both ALK-3 and ALK-6 signaling. TbetaRIII associated with ALK-3 primarily through their extracellular domains, whereas its interaction with ALK-6 required both the extracellular and cytoplasmic domains. ALK-3 plays an essential role in the formation of embryonic ventral abdominal wall, and abrogation of BMP signaling activity due to gene mutations in its signaling components could be one of the underlying causes of omphalocele at birth. The type IA BMP receptor, ALK-3 was specifically required at mid-gestation for normal development of the trabeculae, compact myocardium, interventricular septum, and endocardial cushion. Cardiac muscle lacking ALK-3 was specifically deficient in expressing TGFbeta2, an established paracrine mediator of cushion morphogenesis. Hence, ALK-3 is essential, beyond just the egg cylinder stage, for myocyte-dependent functions and signals in cardiac organogenesis.