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人 ANXA5 基因ORF全长cDNA克隆(表达载体), N-His 标签

  • Ferret TNF-alpha/TNFA/TNFSF2 Gene Plasmid Map 5629
产品数据评论相关产品实验方法
 ANXA5 cDNA Clone产品信息
NCBI注册码:NM_001154.3
参考序列ORF长度:1008 bp
cDNA基因描述:Full length Clone DNA of Homo sapiens annexin A5 with N terminal His tag.
分子别称:PP4, ANX5, ENX2, ANXA5
分子种属:Human
载体:pCMV3-N-His
质粒:pCMV3-His-ANXA5
限制性酶切位点:KpnI + XbaI(6kb+1.01kb)
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with ANXA5 qPCR primers for gene expression analysis, HP100477 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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参考资料
  • Cederholm A, et al. (2007) Annexin A5 as a novel player in prevention of atherothrombosis in SLE and in the general population. Ann N Y Acad Sci. 1108: 96-103.
  • Schlaepfer DD, et al. (1992) Inhibition of Protein Kinase C by AnnexinⅤ. Biochemistry. 31: 1886-91.
  • Vermes I, et al. (1995) A novel assay for apoptosis-flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin Ⅴ. J Immunol Methods. 184 (1): 39-51.
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    货号: HG10448-NH
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