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人 Azurocidin / CAP37 基因ORF全长cDNA克隆(表达载体)

  • Human AZU1 / HBP Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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 AZU1 cDNA Clone产品信息
NCBI注册码:NM_001700.3
参考序列ORF长度:756bp
cDNA基因描述:Full length Clone DNA of Homo sapiens azurocidin 1.
分子别称:AZU, HBP, NAZC, AZAMP, CAP37, HUMAZUR, AZU1
分子种属:Human
载体:pCMV3-untagged
质粒:pCMV3-AZU1
限制性酶切位点:KpnI + XbaI (6.1kb + 0.76kb)
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 165C>A not causing the amino acid variation.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with AZU1 qPCR primers for gene expression analysis, HP100629 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Ampicillin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
 AZU1 基因质粒图
Human AZU1 / HBP Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
Product nameProduct name
研究背景

Azurocidin (AZU1), also known as heparin-binding protein (HBP) or cationic antimicrobial protein 37 (CAP37), is an azurophil granule antibiotic protein, with monocyte chemotactic and antibacterial activity. The Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. Azurocidin is a member of the serine protease family that includes Cathepsin G, neutrophil elastase (NE), and proteinase 3 (PR3), however, Azurocidin is not a serine proteinase since the active site serine and histidine residues are replaced. Neutrophils arriving first at sites of inflammation release Azurocidin, which acts in a paracrine fashion on endothelial cells causing the development of intercellular gaps and allowing leukocyte extravasation. It thus be regarded as a reasonable therapeutic target for a variety of inflammatory disease conditions.

参考资料
  • Lindmark A, et al. (1999) Characterization of the biosynthesis, processing, and sorting of human HBP/CAP37/azurocidin. J Leukoc Biol. 66(4): 634-43.
  • Heinzelmann M, et al. (2001) Heparin binding protein (CAP37) differentially modulates endotoxin-induced cytokine production. Int J Surg Investig. 2(6): 457-66.
  • Soehnlein O, et al. (2005) Neutrophil-derived heparin-binding protein (HBP/CAP37) deposited on endothelium enhances monocyte arrest under flow conditions. J Immunol. 174(10): 6399-405.
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    货号: HG10660-UT
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