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人 Angiogenin / RNASE5 基因ORF全长cDNA克隆(表达载体), C-His 标签

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Human ANG cDNA Clone产品信息
NCBI注册码:NM_001097577.2
参考序列ORF长度:444bp
cDNA基因描述:Full length Clone DNA of Homo sapiens angiogenin, ribonuclease, RNase A family, 5 with C terminal His tag.
分子别称:ALS9, HEL168, RNASE4, RNASE5, MGC22466, MGC71966, ANG
分子种属:Human
载体:pCMV3-C-His
质粒:
限制性酶切位点:
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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