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人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签

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Human ANGPTL4 cDNA Clone产品信息
NCBI注册码:NM_139314.1
参考序列ORF长度:1221bp
cDNA基因描述:Full length Clone DNA of Homo sapiens angiopoietin-like 4, transcript variant 1 with N terminal Flag tag.
分子别称:NL2, ARP4, FIAF, PGAR, HFARP, pp1158, ANGPTL2
分子种属:Human
载体:pCMV3-SP-N-FLAG
质粒:
限制性酶切位点:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签 on other vectors
人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG10563-ACG1950
人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG10563-ACR1950
人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10563-CF1770
人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-His 标签HG10563-CH1770
人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG10563-CM1770
人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG10563-CY1770
人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA(克隆载体)HG10563-M760
人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10563-M-F1770
人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG10563-NF1770
人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-His 标签HG10563-NH1770
人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG10563-NM1770
人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG10563-NY1770
人 ANGPTL4 / angiopoietin-like 蛋白 4 transcript variant 1 基因ORF全长cDNA克隆(表达载体)HG10563-UT1770
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研究背景

ANGPTL4, also known as ANGPTL2, is a protein with hypoxia-induced expression in endothelial cells. It contains 1 fibrinogen C-terminal domain and is expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. ANGPTL4 inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. It may act as a regulator of angiogenesis and modulate tumorigenesis. It inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. It may also exert a protective function on endothelial cells through an endocrine action. ANGPTL4 is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.

参考资料
  • Lichtenstein L, et al. (2010) Angptl4 Protects against Severe Proinflammatory Effects of Saturated Fat by Inhibiting Fatty Acid Uptake into Mesenteric Lymph Node Macrophages. Cell metabolism. 12(6): 580-92.
  • Terada S, et al. (2011) Escaping Anoikis through ROS: ANGPTL4 controls integrin signaling through Nox1. Cancer Cell. 19(3):297-9.
  • Zhu PC, et al. (2011) Angptl4 protein elevates the prosurvival intracellular O2(-):H2O2 ratio and confers anoikis resistance to tumors. Cancer Cell. 19(3):401-15.
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