CD86 cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG10699-CF
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All CD86 Reagents
CRO Services
Product recommended

All CD86 Reagents
CD86 cDNA ORF Clone, Human, C-DDK (Flag®) tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
972 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human CD86 molecule (CD86), transcript variant 2 with C terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-FLAG
限制性酶切位点
KpnI + XbaI (6kb + 1.02kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
CD86 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CD86 cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images
Human CD86/B7-2 Gene Expression validated Image 1599
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
other Green fluorescent protein / GFP Gene Plasmid Map 5609
CD86 cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
B7-2 cDNA ORF Clone, Human;B7.2 cDNA ORF Clone, Human;B70 cDNA ORF Clone, Human;CD28LG2 cDNA ORF Clone, Human;LAB72 cDNA ORF Clone, Human
CD86 Background Information

CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD86 antigen
References
  • Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.
  • Rau FC, et al. (2009) B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol. 183(12): 7661-71.
  • Dai ZS, et al. (2009) Defective expression and modulation of B7-2/CD86 on B cells in B cell chronic lymphocytic leukemia. Int J Hematol. 89(5): 656-63.
  • CD86 cDNA ORF Clone, Rhesus, Related Products

    Product Name Catalog#(PDF)
    CD86 cDNA ORF Clone in Cloning Vector, Rhesus CG90270-G
    CD86 cDNA ORF Clone, Rhesus, untagged CG90270-UT
    CD86 cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90270-ACG
    CD86 Lentiviral cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90270-ACGLN
    CD86 cDNA ORF Clone, Rhesus, C-OFPSpark® tag CG90270-ACR
    CD86 cDNA ORF Clone, Rhesus, C-DDK (Flag®) tag CG90270-CF
    CD86 cDNA ORF Clone, Rhesus, N-DDK (Flag®) tag CG90270-NF
    CD86 cDNA ORF Clone, Rhesus, C-His tag CG90270-CH
    CD86 cDNA ORF Clone, Rhesus, N-His tag CG90270-NH
    CD86 cDNA ORF Clone, Rhesus, C-HA tag CG90270-CY
    CD86 cDNA ORF Clone, Rhesus, N-HA tag CG90270-NY
    CD86 cDNA ORF Clone, Rhesus, C-Myc tag CG90270-CM
    CD86 cDNA ORF Clone, Rhesus, N-Myc tag CG90270-NM

    CD86 cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    CD86 cDNA ORF Clone in Cloning Vector, Rat RG80025-M
    CD86 cDNA ORF Clone, Rat, untagged RG80025-UT
    CD86 cDNA ORF Clone, Rat, C-GFPSpark® tag RG80025-ACG
    CD86 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80025-ACGLN
    CD86 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80025-ACR
    CD86 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80025-CF
    CD86 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80025-NF
    CD86 cDNA ORF Clone, Rat, C-His tag RG80025-CH
    CD86 cDNA ORF Clone, Rat, N-His tag RG80025-NH
    CD86 cDNA ORF Clone, Rat, C-HA tag RG80025-CY
    CD86 cDNA ORF Clone, Rat, N-HA tag RG80025-NY
    CD86 cDNA ORF Clone, Rat, C-Myc tag RG80025-CM
    CD86 cDNA ORF Clone, Rat, N-Myc tag RG80025-NM
    CD86 qPCR Primer Pairs, Rat RP300024

    CD86 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    CD86 cDNA ORF Clone in Cloning Vector, Mouse MG50068-M
    CD86 cDNA ORF Clone, Mouse, untagged MG50068-UT
    CD86 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50068-ACG
    CD86 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50068-ACGLN
    CD86 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50068-ACR
    CD86 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50068-CF
    CD86 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50068-NF
    CD86 cDNA ORF Clone, Mouse, C-His tag MG50068-CH
    CD86 cDNA ORF Clone, Mouse, N-His tag MG50068-NH
    CD86 cDNA ORF Clone, Mouse, C-HA tag MG50068-CY
    CD86 cDNA ORF Clone, Mouse, N-HA tag MG50068-NY
    CD86 cDNA ORF Clone, Mouse, C-Myc tag MG50068-CM
    CD86 cDNA ORF Clone, Mouse, N-Myc tag MG50068-NM
    CD86 qPCR Primer Pairs, Mouse MP200099

    CD86 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    CD86 cDNA ORF Clone in Cloning Vector, Human HG10699-G
    CD86 cDNA ORF Clone, Human, untagged HG10699-UT
    CD86 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10699-ACGLN
    CD86 cDNA ORF Clone, Human, C-GFPSpark® tag HG10699-ACG
    CD86 cDNA ORF Clone, Human, C-OFPSpark® tag HG10699-ACR
    CD86 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10699-NF
    CD86 cDNA ORF Clone, Human, C-His tag HG10699-CH
    CD86 cDNA ORF Clone, Human, N-His tag HG10699-NH
    CD86 cDNA ORF Clone, Human, C-HA tag HG10699-CY
    CD86 cDNA ORF Clone, Human, N-HA tag HG10699-NY
    CD86 cDNA ORF Clone, Human, C-Myc tag HG10699-CM
    CD86 cDNA ORF Clone, Human, N-Myc tag HG10699-NM
    CD86 qPCR Primer Pairs, Human HP100099
    info info
    添加购物车成功! 添加购物车失败!