Bcl-XL cDNA ORF Clone, Human, C-His tag

Cat: HG10455-CH
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Bcl-XL cDNA ORF Clone, Human, C-His tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
702 bp
描述
Full length Clone DNA of Human BCL2-like 1 with C terminal His tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-His
标签序列
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Bcl-XL cDNA ORF Clone, Human, C-His tag Alternative Names
Bcl-X cDNA ORF Clone, Human;bcl-xL cDNA ORF Clone, Human;BCL-XL/S cDNA ORF Clone, Human;bcl-xS cDNA ORF Clone, Human;BCL2L cDNA ORF Clone, Human;BCLX cDNA ORF Clone, Human;BCLXL cDNA ORF Clone, Human;BCLXS cDNA ORF Clone, Human;PPP1R52 cDNA ORF Clone, Human
Bcl-XL Background Information

B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1) , belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is chareacterized as important factors in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.

Full Name
BCL2-like 1
References
  • Vander Heiden MG, et al. (1997) Bcl-xL Regulates the Membrane Potential and Volume Homeostasis of Mitochondria. Cell. 91 (5): 627-37.
  • Muchmore SW, et al. (1996) X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death. Nature. 381: 335-341.
  • SharoffEH, et al. (2007) Bcl-2 family members regulate anoxia-induced cell death. Antioxid Redox Signal. 9 (9) :1405-9.
  • Zhou F, et al. (2011) Bcl-2 and Bcl-xL play important roles in the crosstalk between autophagy and apoptosis. FEBS J. 278 (3): 403-13.
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    Bcl-XL cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    Bcl-XL cDNA ORF Clone in Cloning Vector, Human HG10455-M
    Bcl-XL cDNA ORF Clone, Human, untagged HG10455-UT
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    Bcl-XL Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10455-ACGLN
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    Bcl-XL cDNA ORF Clone, Human, N-OFPSpark® tag HG10455-ANR
    Bcl-XL cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10455-CF
    Bcl-XL cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10455-NF
    Bcl-XL cDNA ORF Clone, Human, N-His tag HG10455-NH
    Bcl-XL cDNA ORF Clone, Human, C-HA tag HG10455-CY
    Bcl-XL cDNA ORF Clone, Human, N-HA tag HG10455-NY
    Bcl-XL cDNA ORF Clone, Human, C-Myc tag HG10455-CM
    Bcl-XL cDNA ORF Clone, Human, N-Myc tag HG10455-NM
    Bcl-XL qPCR Primer Pairs, Human HP100024
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