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人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签

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Human BIRC5 cDNA Clone产品信息
NCBI注册码:NM_001168.2
参考序列ORF长度:429bp
cDNA基因描述:Full length Clone DNA of Homo sapiens baculoviral IAP repeat-containing 5, transcript variant 1 with C terminal Flag tag.
分子别称:API4, EPR-1
分子种属:Human
载体:pCMV3-C-FLAG
质粒:pCMV3-BIRC5-Flag
限制性酶切位点:KpnI + XbaI (6kb + 0.47kb)
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human BIRC5 基因质粒图
Human BIRC5 transcript variant 1 natural ORF mammalian expression plasmid, C-Flag tag
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签 on other vectors
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG10356-ACG1950
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG10356-ACR1950
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG10356-ANG1950
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG10356-ANR1950
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10356-CF1770
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-His 标签HG10356-CH1770
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG10356-CM1770
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG10356-CY1770
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA(克隆载体)HG10356-M760
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体)HG10356-M-N1770
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG10356-NF1770
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-His 标签HG10356-NH1770
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG10356-NM1770
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG10356-NY1770
人 Survivin/BIRC5/API4 transcript variant 1 基因ORF全长cDNA克隆(表达载体)HG10356-UT1770
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研究背景

BIRC5, also known as Survivin and EPR-1, is a member of the IAP family. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but BIRC5 has only a single BIR domain. It is expressed cell cycle-dependently and highly expressed at mitosis. As a multitasking protein, BIRC5 has dual roles in promoting cell proliferation and preventing apoptosis. Survivin is a component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Survivin acts as an important regulator of the localization of this complex. It may counteract a default induction of apoptosis in G2/M phase.

参考资料
  • Altieri DC. 1994, J Biol Chem. 269 (5): 3139-42.
  • Bouchard BA. et al., 2002, Thromb Haemost. 86 (4): 1133-5.
  • Yao XQ. et al., 2004, World J Gastroenterol. 10 (9): 1262-7.
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