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人 Biglycan 基因ORF全长cDNA克隆(表达载体), N-His 标签

  • Human Biglycan natural ORF mammalian expression plasmid, N-His tag
产品数据评论相关产品实验方法
 BGN cDNA Clone产品信息
NCBI注册码:NM_001711.3
参考序列ORF长度:1107bp
cDNA基因描述:Full length Clone DNA of Homo sapiens biglycan with N terminal His tag.
分子别称:PGI, DSPG1, PG-S1, SLRR1A, BGN
分子种属:Human
载体:pCMV3-SP-N-His
质粒:pCMV3-His-BGN
限制性酶切位点:KpnI + XbaI (6kb + 1.14kb)
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列信息:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 576C/T not causing the amino acid variation.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with BGN qPCR primers for gene expression analysis, HP100476 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
 BGN 基因质粒图
Human Biglycan natural ORF mammalian expression plasmid, N-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Product nameProduct name
研究背景

Biglycan, also known as PG-S1 and BGN, is a a small leucine-rich repeat proteoglycan (SLRP). It can be detected in a variety of extracellular matrix tissues, including bone, cartilage and tendon. Biglycan consists of a protein core containing leucine-rich repeat regions and two glycosaminoglycan (GAG) chains consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS). Non-glycanated forms of biglycan (no GAG chains) increase with age in human articular cartilage. Biglycan interacts with collagen, both via the core protein and GAG chains. Biglycan plays a role in the mineralisation of bone. Biglycan core protein binds to the growth factors BMP-4 and influences its bioactivity.

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货号: HG10447-NH
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