Biglycan cDNA ORF Clone, Human, N-His tag

Cat: HG10447-NH

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Biglycan cDNA ORF Clone, Human, N-His tag 基本信息

基因

种属
Human
NCBI 参考序列号
参考序列ORF长度
1107 bp
序列描述
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 576C/T not causing the amino acid variation.
描述
Full length Clone DNA of Human biglycan with N terminal His tag.

质粒

启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + XbaI (6kb + 1.14kb)
标签序列
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.

筛选

抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression

储存 & 运输

运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

Biglycan cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Biglycan cDNA ORF Clone, Human, N-His tag Validated Images

Rhesus CD3d/CD3 delta Gene Plasmid Map 5615

Biglycan cDNA ORF Clone, Human, N-His tag Alternative Names

DSPG1 cDNA ORF Clone, Human;PG-S1 cDNA ORF Clone, Human;PGI cDNA ORF Clone, Human;SLRR1A cDNA ORF Clone, Human

Biglycan Background Information

Biglycan, also known as PG-S1 and BGN, is a a small leucine-rich repeat proteoglycan (SLRP). It can be detected in a variety of extracellular matrix tissues, including bone, cartilage and tendon. Biglycan consists of a protein core containing leucine-rich repeat regions and two glycosaminoglycan (GAG) chains consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS). Non-glycanated forms of biglycan (no GAG chains) increase with age in human articular cartilage. Biglycan interacts with collagen, both via the core protein and GAG chains. Biglycan plays a role in the mineralisation of bone. Biglycan core protein binds to the growth factors BMP-4 and influences its bioactivity.

Full Name
biglycan
References
  • Fisher L.W., et al.,(1989), Deduced protein sequence of bone small proteoglycan I (biglycan) shows homology with proteoglycan II (decorin) and several nonconnective tissue proteins in a variety of species. J. Biol. Chem. 264:4571-4576.
  • Fisher L.W., et al., (1991), Human biglycan gene. Putative promoter, intron-exon junctions, and chromosomal localization.J. Biol. Chem. 266:14371-14377.
  • Mallon A.-M., et al.,(2000), Comparative genome sequence analysis of the Bpa/Str region in mouse and man.Genome Res. 10:758-775.
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