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人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体), C-Myc 标签

产品数据评论相关产品实验方法
Human BTK cDNA Clone产品信息
NCBI注册码:NM_000061.2
参考序列ORF长度:1980bp
cDNA基因描述:Full length Clone DNA of Homo sapiens Bruton agammaglobulinemia tyrosine kinase with C terminal Myc tag.
分子别称:AT, ATK, BPK, XLA, IMD1, AGMX1, PSCTK1, MGC126261, MGC126262
分子种属:Human
载体:pCMV3-C-Myc
质粒:pCMV3-BTK-Myc
限制性酶切位点:KpnI + NotI (6kb + 2.03kb)
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列信息:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1899C/T not causing the amino acid variation.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human BTK 基因质粒图
Human Bruton Tyrosine Kinase / BTK Kinase ORF mammalian expression plasmid, C-Myc tag
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体), C-Myc 标签 on other vectors
人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG10578-ACG2070
人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG10578-ACR2070
人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG10578-ANG2070
人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG10578-ANR2070
人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10578-CF1890
人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体), C-His 标签HG10578-CH1890
人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG10578-CM1890
人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG10578-CY1890
人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA(克隆载体)HG10578-M920
人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG10578-NF1890
人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体), N-His 标签HG10578-NH1890
人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG10578-NM1890
人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG10578-NY1890
人 Bruton Tyrosine Kinase / BTK Kinase 基因ORF全长cDNA克隆(表达载体)HG10578-UT1890
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研究背景

Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.

参考资料
  • Hashimoto S, et al. (1996) Identification of Bruton's tyrosine kinase (Btk) gene mutations and characterization of the derived proteins in 35 X-linked agammaglobulinemia families: a nationwide study of Btk deficiency in Japan. Blood. 88(2): 561-73.
  • Ohta Y, et al. (1994) Genomic organization and structure of Bruton agammaglobulinemia tyrosine kinase: localization of mutations associated with varied clinical presentations and course in X chromosome-linked agammaglobulinemia. PNAS. 91(19): 9062-6.
  • Smith C, et al. (1994) Expression of Bruton's agammaglobulinemia tyrosine kinase gene, BTK, is selectively down-regulated in T lymphocytes and plasma cells. The Journal of Immunology. 152(2): 557-65.
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