Human CART HEK293 Overexpression Lysate

Cat: 13240-HCCHL

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Human CART HEK293 Overexpression Lysate 产品信息

Product Description
This Human CART overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CART protein (Cat: 13240-HCCH) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Species
Human
Sequence Information
A DNA sequence encoding the human CARTPT (NP_004282.1) (Met1-Leu116) was expressed with five amino acids (DDDDK) at the C-terminus.
Molecule Mass
The recombinant human CARTPT consists of 95 amino acids and predicts a molecular mass of 10.6 KDa. It migrates as an approximately 11 KDa band in SDS-PAGE under reducing conditions.

Human CART HEK293 Overexpression Lysate Usage Guide

Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human CART HEK293 Overexpression Lysate Alternative Names

Human CART Overexpression Lysate

CART Background Information

Full Name
CART prepropeptide

Human Overexpression Lysate

Product Name Cat.No.
Human CART HEK293 Overexpression Lysate 13240-H05HL
info info
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