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人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-HA 标签

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     CBR3 cDNA Clone产品信息
    NCBI注册码:BC002812
    参考序列ORF长度:834bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens carbonyl reductase 3 with N terminal HA tag.
    分子别称:hCBR3, SDR21C2
    分子种属:Human
    载体:pCMV3-N-HA
    质粒:
    限制性酶切位点:
    Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with CBR3 qPCR primers for gene expression analysis, HP103266 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-HA 标签 on other vectors
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG14632-ACG1950
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG14632-ACR1950
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG14632-ANG1950
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG14632-ANR1950
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG14632-CF1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), C-His 标签HG14632-CH1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG14632-CM1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG14632-CY1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA(克隆载体)HG14632-G760
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG14632-NF1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-His 标签HG14632-NH1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG14632-NM1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG14632-NY1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体)HG14632-UT1770
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    研究背景

    CBR3, also known as hCBR3, belongs to the short-chain dehydrogenases/reductases (SDR) family. CBR3 is expressed in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis. It is a monomeric NADPH-dependent oxidoreductase and is closely linked to another carbonyl reductase gene – CBR1. CBR3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. It has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).

    参考资料
  • Lakhman SS. et al., 2005. Drug Metab Dispos. 33 (2): 254-7.
  • Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.
  • Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903.
  • Size / Price
    货号: HG14632-NY
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