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人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-Myc 标签

    产品数据评论相关产品实验方法
     CBR3 cDNA Clone产品信息
    NCBI注册码:BC002812
    参考序列ORF长度:834bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens carbonyl reductase 3 with N terminal Myc tag.
    分子别称:hCBR3, SDR21C2
    分子种属:Human
    载体:pCMV3-N-Myc
    质粒:
    限制性酶切位点:
    Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with CBR3 qPCR primers for gene expression analysis, HP103266 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    Myc Tag Info

    A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

    The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-Myc 标签 on other vectors
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG14632-ACG1950
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG14632-ACR1950
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG14632-ANG1950
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG14632-ANR1950
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG14632-CF1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), C-His 标签HG14632-CH1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG14632-CM1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG14632-CY1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA(克隆载体)HG14632-G760
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG14632-NF1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-His 标签HG14632-NH1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG14632-NM1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG14632-NY1770
    人 CBR3 / HCBR3 / Carbonyl Reductase 3 基因ORF全长cDNA克隆(表达载体)HG14632-UT1770
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    研究背景

    CBR3, also known as hCBR3, belongs to the short-chain dehydrogenases/reductases (SDR) family. CBR3 is expressed in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis. It is a monomeric NADPH-dependent oxidoreductase and is closely linked to another carbonyl reductase gene – CBR1. CBR3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. It has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).

    参考资料
  • Lakhman SS. et al., 2005. Drug Metab Dispos. 33 (2): 254-7.
  • Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.
  • Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903.
  • Size / Price
    货号: HG14632-NM
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