( We provide with CCDC47 qPCR primers for gene expression analysis, HP101983 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 CCDC47 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG13274-ACG|
|人 CCDC47 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG13274-ACR|
|人 CCDC47 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG13274-CF|
|人 CCDC47 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG13274-CH|
|人 CCDC47 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG13274-CM|
|人 CCDC47 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG13274-CY|
|人 CCDC47 基因ORF全长cDNA(克隆载体)||HG13274-G|
|人 CCDC47 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG13274-NF|
|人 CCDC47 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG13274-NH|
|人 CCDC47 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG13274-NM|
|人 CCDC47 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG13274-NY|
|人 CCDC47 基因ORF全长cDNA克隆(表达载体)||HG13274-UT|
CCDC47 gene is expressed at high level. The gene contains 16 distinct gt-ag introns. Transcription produces 9 different mRNAs, 6 alternatively spliced variants and 3 unspliced forms. There are 3 probable alternative promotors, 3 non overlapping alternative last exons and 8 validated alternative polyadenylation sites. The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of a cassette exon, overlapping exons with different boundaries. Functionally, CCDC47 gene has been proposed to participate in processes such as calcium ion homeostasis, embryo development, ER overload response and post-embryonic development. CCDC47 are expected to have molecular function (calcium ion binding) and to localize in various compartments (membrane, endoplasmic reticulum, integral to membrane, microsome).